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Journal of Clinical Immunology

, Volume 21, Issue 2, pp 116–125 | Cite as

IL-12 as Well as IL-2 Upregulates CCR5 Expression on T Cell Receptor-Triggered Human CD4+ and CD8+ T Cells

  • Yi-Fu Yang
  • Michio Tomura
  • Masayuki Iwasaki
  • Takao Mukai
  • Ping Gao
  • Shiro Ono
  • Jian-Ping Zou
  • Gene M. Shearer
  • Hiromi Fujiwara
  • Toshiyuki Hamaoka
Article

Abstract

The expression of chemokine receptors on leukocytes is related to their activation state. However, the exact mechanism underlying the induction of each chemokine receptor is poorly understood. Here, we investigated how CCR5, a chemokine receptor implicated in T cell trafficking and HIV infection, is induced in human T cells. CCR5 was marginally detected on a freshly prepared human peripheral blood mononuclear cell (PBMC) population. Long-term (8-day) stimulation of PBMC with IL-2 resulted in high levels of CCR5 expression on T cells. IL-12 failed to induce CCR5 on T cells in such a directly stimulated PBMC population. Stimulation of PBMC T cells with anti-CD3 plus anti-CD28 induced detectable albeit very low levels of CCR5 along with the induction of IL-12 receptor. However, these TCR-triggered T cells expressed much higher levels of CCR5 when stimulated with IL-12. Although IL-2 also induced CCR5 expression, CCR5 expression was more potent in IL-12 than IL-2 stimulation. These results indicate that, in addition to IL-2, IL-12 plays an important role in the induction of CCR5 expression on T cells, particularly TCR-triggered T cells.

IL-12 IL-2 chemokine receptor CCR5 T cells 

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Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Yi-Fu Yang
    • 1
  • Michio Tomura
    • 1
  • Masayuki Iwasaki
    • 1
  • Takao Mukai
    • 1
  • Ping Gao
    • 1
  • Shiro Ono
    • 1
  • Jian-Ping Zou
    • 2
  • Gene M. Shearer
    • 2
  • Hiromi Fujiwara
    • 1
  • Toshiyuki Hamaoka
    • 1
  1. 1.Department of Oncology (C6), Biomedical Research CenterOsaka University Graduate School of MedicineOsakaJapan
  2. 2.Experimental Immunology Branch, National Cancer InstituteNational Institutes of HealthBethesda

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