, Volume 38, Issue 3, pp 409–413 | Cite as

Effects of Ozone Fumigation on Photosynthesis and Membrane Permeability in Leaves of Spring Barley, Meadow Fescue, and Winter Rape

  • A. Plażek
  • M. Rapacz
  • A. Skoczowski


Seedlings of spring barley, meadow fescue, and winter rape were fumigated with 180 μg kg−1 of ozone for 12 d, and effect of O3 on photosynthesis and cell membrane permeability of fumigated plants was determined. Electrolyte leakage and chlorophyll fluorescence were measured after 6, 9, and 12 d of fumigation, while net photosynthetic rate (PN) and stomatal conductance (gs) were measured 9 d after the start of ozone exposure. O3 treatment did not change membrane permeability in fescue and barley leaves, while in rape a significant decrease in ion leakage was noted within the whole experiment. O3 did not change the photochemical efficiency of photosystem 2 (PS2), i.e., Fv/Fm, and the initial fluorescence (F0). The values of half-rise time (t1/2) from F0 to maximal fluorescence (Fm) decreased in fescue and barley after 6 and 9 d of fumigation. PN decreased significantly in ozonated plants, in the three species. The greatest decrease in PN was observed in ozonated barley plants (17 % of the control). The ozone-induced decrease in PN was due to the closure of stomata. Rape was more resistant to ozone than fescue or barley. Apparently, the rape plants show a large adaptation to ozone and prevent loss of membrane integrity leading to ion leakage.

Brassica napus var. oleifera, chlorophyll fluorescence electrolyte leakage Festuca pratensis Hordeum vulgare net photosynthetic rate ozone stomatal conductance 


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  1. Baszyński, T., Wajda, L., Król, M., Wolińska, D., Krupa, Z., Tukendorf, A.: Photosynthetic activities of cadmium-treated tomato plants.-Physiol. Plant. 48: 365-370, 1980.CrossRefGoogle Scholar
  2. Björkman, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence characteristics among vascular plants of diverse origin.-Planta 170: 489-504, 1987.CrossRefPubMedGoogle Scholar
  3. Farage, P.K., Long, S.P.: The effects of O3 fumigation during leaf development on photosynthesis of wheat and pea: An in vivo analysis.-Photosynth. Res. 59: 1-7, 1999.CrossRefGoogle Scholar
  4. Farage, P.K., Long, S.P., Lechner, E.G., Baker, N.R.: Sequence of changes within the photosynthetic apparatus of wheat following short-term exposure to ozone.-Plant Physiol. 95: 529-535, 1991.CrossRefPubMedPubMedCentralGoogle Scholar
  5. Guidi, L., Bongi, G., Ciompi, S., Soldatini, G.F.: In Vicia faba leaves photoinhibition from ozone fumigation in light precedes a decrease in quantum yield of functional PS2 centres.-J. Plant Physiol. 154: 167-172, 1999.CrossRefGoogle Scholar
  6. Koch, J.R., Scherzer, A.J., Eshita, S.M., Davis, K.R.: Ozone sensitivity in hybrid poplar is correlated with a lack of defense-gene activation.-Plant Physiol. 118: 1243-1252, 1998.CrossRefGoogle Scholar
  7. Krause, G.H., Weis, E.: Chlorophyll fluorescence as a tool in plant physiology.-Photosynth. Res. 5: 139-157, 1984.CrossRefPubMedGoogle Scholar
  8. Krupa, Z., Baranowska, M., Orzoł, D.: Can anthocyanins be considered as heavy metal stress indicator in higher plants?-Acta Physiol. Plant. 18: 147-151, 1996.Google Scholar
  9. Lichtenthaler, H.K.: Vegetation stress: an introduction to the stress concept in plants.-J. Plant Physiol. 148: 4-14, 1996.CrossRefGoogle Scholar
  10. Lo, S.C.C., Nicholson, R.L.: Reduction of light-induced anthocyanin accumulation in inoculated sorghum mesocotyls-Implications for a compensatory role in the defense response.-Plant Physiol. 116: 979-989, 1998.CrossRefPubMedPubMedCentralGoogle Scholar
  11. McKown, R., Kuroki, G., Warren, G.: Cold responses of Arabidopsis mutants impaired in freezing tolerance.-J. exp. Bot. 47: 1919-1925, 1996.CrossRefGoogle Scholar
  12. Miller, J.D., Arteca, R.N., Pell, E.J.: Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis.-Plant Physiol. 120: 1015-1023, 1999.CrossRefPubMedPubMedCentralGoogle Scholar
  13. Reichenauer, T.G., Goodmann, B.A., Kostecki, P., Soja, G.: Ozone sensitivity in Triticum durum and T. aestivum with respect to leaf injury, photosynthetic activity and free radical content.-Physiol. Plant. 104: 681-686, 1998.CrossRefGoogle Scholar
  14. Reid, C.D., Fiscus, E.L., Burkey, K.O.: Combined effects of chronic ozone and elevated CO2 on Rubisco activity and leaf components in soybean (Glycine max).-J. exp. Bot. 49: 1999-2011, 1998.Google Scholar
  15. Skoczowski, A., Pieńkowski, S., Niewiadomska, E., Miszalski, Z.: Phytotron chambers for plant exposure to ozone.-Arch. Environ. Protection 26: 129-135, 2000.Google Scholar
  16. Soja, G., Pfeifer, U., Soja, A.M.: Photosynthetic parameters as early indicators of ozone injury in apple leaves.-Physiol. Plant. 104: 639-645, 1998.CrossRefGoogle Scholar
  17. Soldatini, G.F., Lorenzini, G., Filippi, F., Nali, C., Guidi, L.: Photosynthesis of two poplar clones under long-term exposure to ozone.-Physiol. Plant. 104: 707-712, 1998a.CrossRefGoogle Scholar
  18. Soldatini, G.F., Nali, C., Guidi, L., Lorenzini, G.: Photosynthesis of Hedera canariensis var. azorica variegated leaves as affected by ozone.-Photosynthetica 35: 247-253, 1998b.CrossRefGoogle Scholar
  19. Solecka, D., Boudet, A.M., Kacperska, A.: Phenylpropanoid and anthocyanin changes in low-temperature treated winter oilseed rape leaves.-Plant Physiol. Biochem. 37: 491-496, 1999.CrossRefGoogle Scholar
  20. Yoshida, S., Uemura, M.: Responses of the plasma membrane to cold acclimation and freezing stress.-In: Larsson, C., Moller, I.M. (ed.): The Plasma Membrane. Structure, Function and Molecular Biology. Pp. 293-319. Springer-Verlag, Berlin-Heidelberg 1990.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • A. Plażek
    • 1
  • M. Rapacz
    • 1
  • A. Skoczowski
    • 2
  1. 1.Department of Plant Physiology, Faculty of AgricultureAgricultural University of KrakówKrakówPoland
  2. 2.F. Górski's Department of Plant PhysiologyPolish Academy of SciencesKrakówPoland

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