Effect of oleoyl‐esterone treatment on the expression of β1- β2‐ and β3‐adrenoreceptors in rat adiopose tissues
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Adult female rats received a constant i.v. infusion of oleoyl‐estrone (3.5 μmol/kg day) in a lipidic suspension for 14 days. On days 0 (no treatment), 3, 6, 10 and 14, as well as on day 14 for controls (receiving only the lipid); the rats were killed and the expression of the β1‐, β2‐ and β3‐adrenoceptor genes, in brown adipose tissue and in subcutaneous and periovaric white adipose tissue, were measured by RNA protection assay, and compared with that of cyclophyllin. The β3‐adrenoceptor was the most expressed in all adipose tissues, whereas β2 was the less expressed in brown adipose tissue. Oleoyl‐estrone significantly, but moderately, increased the expression of β‐adrenoceptors in the three adipose tissues: β1 increased in subcutaneous, β2 and β3 in periovaric and β3 in brown adipose tissue. Oleoyl‐estrone also decreased β3 expression in subcutaneous white adipose tissue. On day 10, adipocytes isolated from periovaric white adipose tissue of oleoyl‐estrone‐treated rats showed higher cAMP response to an isoproterenol challenge than the controls. The mechanism by which oleoyl‐estrone elicits the wasting of fat reserves could be mediated by adrenergic pathways, at least in part.
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