Journal of Protein Chemistry

, Volume 20, Issue 3, pp 265–271 | Cite as

Proteomic Detection of Changes in Protein Synthesis Induced by NGX6 Transfected in Human Nasopharyngeal Carcinoma Cells

  • Jiang Li
  • Chen Tan
  • Qiu Xiang
  • Xiaomei Zhang
  • Jian Ma
  • Jie-ru Wang
  • Jianbo Yang
  • Wei-fang Li
  • Shou-rong Shen
  • Songping Liang
  • Guiyuan Li
Article

Abstract

In the previous study, we cloned a new gene, named NGX6, related to nasopharyngeal carcinoma (NPC) at 9p. To study its function in the pathogenesis of NPC, we have investigated changes in protein synthesis between NPC cell line HNE1 and that transfected with the gene. Using high-resolution two-dimensional electrophoresis, we found that 22 protein spots showed variations that were significant and reproducible. Analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database searches identified seven proteins that were upregulated and seven proteins that were downregulated. These proteins included Fas, zinc-finger protein (ZNF), RAB, and Ah receptor-interacting protein (AIP). The functional implications of the identified proteins are discussed.

Two-dimensional gel electrophoresis mass spectrometry protein identification 

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Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Jiang Li
    • 1
  • Chen Tan
    • 1
  • Qiu Xiang
    • 1
  • Xiaomei Zhang
    • 1
  • Jian Ma
    • 1
  • Jie-ru Wang
    • 1
  • Jianbo Yang
    • 1
  • Wei-fang Li
    • 1
  • Shou-rong Shen
    • 1
  • Songping Liang
    • 2
  • Guiyuan Li
    • 1
  1. 1.Cancer Research Institute, School of Xiangya MedicineCentral South UniversityChangshaChina
  2. 2.College of Life ScienceHunan Normal UniversityChangshaChina

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