Plant Growth Regulation

, Volume 32, Issue 2–3, pp 307–313 | Cite as

Cytokinin-regulated transcripts in tobacco cell culture

  • S. Schäfer
  • S. Krolzik
  • G.A. Romanov
  • T. Schmülling
Article

Abstract

To study early responses to the hormone cytokinin we identified transcripts in cell culture of Nicotiana tabacum L. Representational difference analysis (RDA) was used to amplify partial cDNAs of genes that were induced or repressed 45 after addition of 5 × 10−7 to the culture medium. Ten different mRNAs whose abundance changed rapidly after cytokinin treatment were identified. Transcripts coding for osmotin, a peroxidase, pectin methyl esterase, a subunit of NADH:ubiquinone oxidoreductase, a chloroplast nucleoid DNA binding protein homologue and a protein of unknown function were upregulated. Increases of transcript abundance occurred as early as 10 following hormone treatment. The transcripts of a homologue of the TA20 gene and two hitherto unknown transcripts without significant homology to known genes were strongly repressed by cytokinin. The data indicate that the regulation of genes that play a role in energy production, defence, cell wall modification and chloroplast development is part of the early response to cytokinin.

Cytokinin Gene regulation Nicotiana tabacum Plant hormones 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • S. Schäfer
    • 1
  • S. Krolzik
    • 1
  • G.A. Romanov
    • 2
  • T. Schmülling
    • 1
  1. 1.Zentrum für Molekularbiologie der Pflanzen (ZMBP), Allgemeine GenetikUniversität TübingenGermany
  2. 2.Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

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