Plant Growth Regulation

, Volume 32, Issue 2–3, pp 107–114 | Cite as

New methods to analyse auxin-induced growth I: Classical auxinology goes Arabidopsis

Article

Abstract

During recent years, genetic approaches have become invaluable tools to analyse signalling chains in plants. The first step to understand auxin signalling is the isolation of signal transduction mutants. In Arabidopsis, screening for auxin resistant plants has identified a number of mutants. However, it is difficult to link the mutated genes to the rapid growth response to auxin. As yet, there is no published method to measure auxin-induced growth at a sufficient temporal resolution. A novel auxanometer described here will close that gap. Hypocotyl segments are immersed in buffer in a flow-through cuvette. A CCD-camera attached to a microscope is used to image markings on the hypocotyl surfaces and to track their movements across the field of view.To illustrate the applicability of this method for analysing mutants we compared the growth responses of tomato wild type with the mutant diageotropica (dgt). We showed that the mutation completely abolished all phases of the rapid growth reaction to applied auxin. This excludes the possibility that the reduced auxin sensitivity reported in the literature is due to a reduced or delayed response. Our technique was modified for use with the tiny Arabidopsis thaliana hypocotyl segments and inflorescence stems. In both systems auxin induced a growth response after a lag phase of 15–20 minutes. The new method will now be used to characterise the physiological effect of the mutations in auxin signalling.

Arabidopsis auxin CCD-auxanometer mutant rapid growth response tomato (dgt mutant) 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Burström HG (1974) What is wrong with auxin research? In: Davies PJ (ed): Plant Growth Substances 1973. Tokyo: Hirokawa, pp 790-797Google Scholar
  2. 2.
    Cosgrove DJ (1999) Enzymes of agents that enhance cell wall extensibility. Annu Rev Plant Physiol Plant Biol 50: 391-417Google Scholar
  3. 3.
    Feldwisch J, Zettl R, Hesse F, Schell J and Palme K (1992) An auxin-binding protein is localized to the plasma membrane of maize coleoptile cells. Identification by photoaffinity labeling and purification of a 23-kDa polypeptide. Proc Natl Acad Sci USA 89: 475-479Google Scholar
  4. 4.
    Fischer C, Lüthen H, Böttger M and Hertel R (1992) Initial transient growth inhibition in maize coleoptiles following auxin application. J. Plant Physiol 141: 88-92Google Scholar
  5. 5.
    Fluhr R (1998) Ethylene perception: from two-component transducers to gene induction. Trends in Plant Sci. 3: 141-146Google Scholar
  6. 6.
    Hertel R (1995) Auxin binding protein is a red herring. J Exp Bot 46: 461-462Google Scholar
  7. 7.
    Hesse T, Feldwisch J, Balshüsemann D, Bauw G, Puype M, Vanderckove J, Löbler M, Klämbt D, Schell J and Palme K (1989) Molecular cloning and structural analysis of a gene from Zea mays L. coding for a putative receptor for the plant hormone auxin. EMBO 8: 2453-2461Google Scholar
  8. 8.
    Kelly MO and Bradford KJ (1986) Insensitivity of the diageotropica tomato mutant to auxin. Plant. Physiol. 82: 713-717Google Scholar
  9. 9.
    Leyser O (1997) Auxin: Lessons from a mutant weed. Physiologia Plantarum 100: 407-414Google Scholar
  10. 10.
    Löbler M and Klämbt D (1985) Auxin binding protein from coleoptile membranes of corn (Zea mays L.). I. Purification by immunological methods and characterization. J Biol Chem 260: 9848-9853Google Scholar
  11. 11.
    Muday GK, Lomax TL and Rayle DL (1995) Characterization of the growth and auxin physiology of roots of the tomato mutant diageotropica. Planta 195: 548-553Google Scholar
  12. 12.
    Napier RM (1995) Towards an understanding of ABP1. J Exp Bot 46: 1787-1795Google Scholar
  13. 13.
    Tietze-Haß E and Dörffling K (1977) Initial phases of Indolylacetic acid induced growth in coleoptile segments of Avena sativa L. Planta 135: 192-195Google Scholar
  14. 14.
    Venis MA (1995) Auxin binding protein1 is a red herring? Oh no it isn't. J Exp Bot 46: 463-465Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  1. 1.Institut für Allgemeine BotanikGermany
  2. 2.Institut für Allgemeine BotanikGermany

Personalised recommendations