Digestive Diseases and Sciences

, Volume 46, Issue 8, pp 1663–1667

Effect of Ischemia–Reperfusion on Bile Canalicular F-Actin Microfilaments in Hepatocytes of Human Liver Allograft

  • Liliane Benkoël
  • Frank Dodero
  • Jean Hardwigsen
  • Pierre Campan
  • Danielle Botta-Fridlund
  • Dominique Lombardo
  • Yves Patrice Le Treut
  • Albert Chamlian
Article

DOI: 10.1023/A:1010693218680

Cite this article as:
Benkoël, L., Dodero, F., Hardwigsen, J. et al. Dig Dis Sci (2001) 46: 1663. doi:10.1023/A:1010693218680

Abstract

We studied and quantified the effect of ischemia–reperfusion on hepatic F-actin on bile canalicular and basolateral membranes in human liver allografts by means of confocal laser scanning microscopy imaging. The phalloidin–FITC staining of F-actin was normal in liver hepatocytes before reperfusion but decreased significantly after reperfusion (by 25% of controls). These results indicate that hepatic F-actin alteration is produced during the reperfusion phase. This modification, probably induced by reactive oxygen species, could impair bile canalicular contraction and tight junction permeability and consequently bile secretion in the postoperative period.

human liver allografts ischemia–reperfusion F-actin confocal laser scanning microscopy 

Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Liliane Benkoël
    • 1
  • Frank Dodero
    • 1
  • Jean Hardwigsen
    • 2
  • Pierre Campan
    • 2
  • Danielle Botta-Fridlund
    • 3
  • Dominique Lombardo
    • 1
  • Yves Patrice Le Treut
    • 2
  • Albert Chamlian
    • 2
  1. 1.Laboratoire de Pathologie Cellulaire et Métabolique du FoieINSERM U 559, Faculté de MédecineMarseille Cedex 05France
  2. 2.Unité de Transplantation HépatiqueHôpital de la ConceptionMarseille Cedex 05France
  3. 3.Service d'Hépato-GastroentérologieHôpital de la ConceptionMarseille Cedex 05France

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