Digestive Diseases and Sciences

, Volume 46, Issue 6, pp 1305–1312 | Cite as

Gender Affects Reperfusion Injury in Rat Liver

  • Antonio Gasbarrini
  • Giovanni Addolorato
  • Cristiana Di Campli
  • Mara Simoncini
  • Salvatore Montemagno
  • Marco Castagneto
  • Cristiano Padalino
  • Paolo Pola
  • Giovanni Gasbarrini


Sex mismatch is a well-known risk factor for chronic rejection of liver allografts, but the mechanisms involved remain unclear. Since experimental observations suggest that female liver is more sensitive to reperfusion injury than male liver, we assessed the influence of gender on oxidative stress. Livers from male and female rats were exposed to warm ischemia and reperfused by an oxygenated buffer. Chemiluminescence was continuously recorded. Reduced and oxidized glutathione and malondialdheyde lactic dehydrogenase values were also determined. Chemiluminescence increased during reperfusion in both groups, but was significantly greater in livers from female rats. Malondyaldheide and lactic dehydrogenase progressively increased in all animals, reaching significantly greater values in female rats. Livers from female rats showed an increase in all the parameters of oxidative stress compared to male animals. A greater susceptibility to reperfusion injury may be evoked as an alternative mechanism to explain the poor outcome of female organ after liver transplantation.

gender ischemia–reperfusion liver injury chemiluminescence 


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Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Antonio Gasbarrini
    • 1
  • Giovanni Addolorato
    • 2
  • Cristiana Di Campli
    • 2
  • Mara Simoncini
    • 2
  • Salvatore Montemagno
    • 3
  • Marco Castagneto
    • 3
  • Cristiano Padalino
    • 2
  • Paolo Pola
    • 1
  • Giovanni Gasbarrini
    • 2
  1. 1.Patologia MedicaCatholic University of RomeRomeItaly
  2. 2.Medicina InternaCatholic University of RomeRomeItaly
  3. 3.Chirurgia dei TrapiantiCatholic University of RomeRomeItaly

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