Plant Molecular Biology

, Volume 46, Issue 3, pp 325–334

The 3′ non-coding region of a C4 photosynthesis gene increases transgene expression when combined with heterologous promoters

  • Shahjahan Ali
  • William C. Taylor
Article

DOI: 10.1023/A:1010669204137

Cite this article as:
Ali, S. & Taylor, W.C. Plant Mol Biol (2001) 46: 325. doi:10.1023/A:1010669204137

Abstract

The Me1 gene of the dicot Flaveria bidentis encodes NADP malic enzyme, which catalyses the decarboxylation reaction of C4 photosynthesis in bundle sheath cells. We have previously shown that the 3′ non-coding region (Me1 3′) controls quantitative expression of the gene. We wondered whether Me1 3′ can increase expression when combined with heterologous promoters. We tested a highly expressed, constitutive promoter, the S4 promoter from subterranean clover stunt virus, and a highly expressed, leaf-specific promoter, the light-harvesting chlorophyll a/b-binding protein gene 3 (Lhcb3) promoter of Arabidopsis thaliana. Promoter-3′-end combinations were tested in transgenic C4Flaveria plants and C3 tobacco. We found that Me1 3′ increased expression of the gusA reporter gene several-fold in leaves of both species in combination with either of the promoters. In both cases Me1 3′ does not alter the expression pattern for either promoter. We conclude that Me1 3′ can be used as a transcription terminator to increase transgene expression in C3 dicot plants.

C4 gene Flaveria bidentis tobacco transcription terminator transgene expression 3′ non-coding region 

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Shahjahan Ali
    • 1
  • William C. Taylor
    • 1
  1. 1.CSIRO Plant IndustryCanberraAustralia

Personalised recommendations