Plant Cell, Tissue and Organ Culture

, Volume 66, Issue 3, pp 217–225 | Cite as

Photoautotrophic growth response of in vitro cultured coffee plantlets to ventilation methods and photosynthetic photon fluxes under carbon dioxide enriched condition

  • Quynh Thi Nguyen
  • Toyoki Kozai
  • Jeongwook Heo
  • Du Xuan Thai


Effects of two ventilation methods (forced and natural) and two photosynthetic photon fluxes (PPF, 150 and 250 μmol m−2 s−1) on the photoautotrophic growth of in vitro cultured coffee (Coffea arabusta) plantlets were investigated. Number of air exchanges was 2.7, 5.9 and 3.9 h−1 for forced low rate, forced high rate and natural ventilation, respectively. Single node cuttings of in vitro cultured coffee plantlets were cultured on Florialite, a mixture of vermiculite and cellulose fibers with high air porosity, emerged in liquid half strength basal MS medium, without sucrose, vitamins and plant growth regulators. The study included 40 days in the in vitro stage and 10 days in the ex vitro stage. Mean fresh and dry weights, leaf area, shoot and root lengths and net photosynthetic rate per plantlet were significantly greater in forced high rate treatments compared with those in natural and forced low rate treatments. PPF had a distinct effect on shoot length suppression and root elongation of coffee plantlets in forced high rate treatments. The control of carbon dioxide concentration inside the culture box according to the plant demand when growing was easy with the forced ventilation method in photoautotrophic micropropagation.

CO2 enriched condition flow rate forced ventilation natural ventilation number of air exchanges photoautotrophic micropropagation 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Quynh Thi Nguyen
    • 1
    • 2
  • Toyoki Kozai
    • 2
  • Jeongwook Heo
    • 3
  • Du Xuan Thai
    • 4
  1. 1.Laboratory of Environmental Control Engineering, Faculty of HorticultureChiba UniversityMatsudo, ChibaJapan
  2. 2.Laboratory of Plant Cell Technology, Institute of Tropical Biology, NCST-VNHo Chi Minh CityVietnam
  3. 3.Research Center for the Development of Advanced HortechnologyChungbuk National University, ChungbukCheongjuRepublic of Korea
  4. 4.Laboratory of Plant Cell Technology, Institute of Tropical Biology, NCST-VNHo Chi Minh CityVietnam

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