Plant Cell, Tissue and Organ Culture

, Volume 66, Issue 1, pp 67–71 | Cite as

Low-cost alternatives for the micropropagation of banana

  • Andrea Kodym
  • Francisco Javier Zapata-Arias


A 90% resource cost reduction in tissue culture of banana was achieved by replacing tissue culture grade sucrose and Gelrite in the medium with locally available commercial sugar and a starch/Gelrite mixture and by using sun light instead of artificial light. The micropropagation of Musa `Grande Naine' by shoot tip culture was used as model. Thirteen commercial sugars from different countries were tested. Best results were achieved using white and light brown sugars with low electrical conductivity. Sugars of cane or sugar beet origin were suitable. Starches of corn or potato could partially substitute for Gelrite and agar. In all experiments, micropropagation rates under natural light conditions were equal to or higher than under the controlled conditions of a growth room with PPFD of 65 μmol m−2 s−1 and a 16-h photoperiod. Plants were exposed to average PPFD levels of 58–96 μmol m−2 s−1 and photoperiods ranged from 8–16 hours.

in vitro culture Musa natural light starch sugar sunlight 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anonymous (1998) Solar illuminated biofactories. Agricell Rep. 31: 27Google Scholar
  2. Bhattacharyya P, Dey S & Bhattacharyya BC (1994) Use of lowcost gelling agents and support matrices for industrial scale plant tissue culture. Plant Cell Tiss. Org. Cult. 37: 15-23CrossRefGoogle Scholar
  3. Bonaobra ZS, Rillo EP & Ebert AW (1994) Effect of table grade sugars on growth and development of coconut (Coco nucifera) embryos in vitro. Presented as poster paper at the 24th International Horticultural Congress, Kyoto International Conference Hall, Kyoto, JapanGoogle Scholar
  4. Ganapathi TR, Mohan JSS, Suprasanna P, Bapat VA & Rao PS (1995) A low-cost strategy for in vitro propagation of banana. Current Sci. 68: 646-649Google Scholar
  5. Kodym A & Zapata-Arias FJ (1999) Natural light as an alternative light source for the in vitro culture of banana (Musa acuminata cv ‘Grande Naine’). Plant Cell Tiss. Org. Cult. 55: 141-145CrossRefGoogle Scholar
  6. Maheshwari SC, Tyagi AK, Malhotra K & Sopory SK (1980) Induction of haploidy from pollen grains in angiosperms-the current status. Theor. Appl. Genet. 58: 193-206CrossRefGoogle Scholar
  7. Marchal J, Sens I & Teisson C (1992) Influence des sucres et de facteurs bioclimatiques sur la culture in vitro du bananier. Fruits 47: 17-24Google Scholar
  8. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497CrossRefGoogle Scholar
  9. Navarro C, Teisson C, Cote F & Ganry J (1994) Effects of light intensity and CO2concentration on growth of banana plants in vitro and subsequent growth following acclimatisation. Sci. Hortic. 60: 41-54CrossRefGoogle Scholar
  10. Van Duren M, Morpurgo R, Dolezel J & Afza R (1996) Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques. Euphytica 88: 25-34CrossRefGoogle Scholar
  11. Van der Salm TPM, Van der Toorn CJG, Haenisch ten Cate CH, Dubois LAM, De Vries DP & Dons HJ (1994) Importance of the iron chelate formula for micropropagation of Rosa hybrida L. ‘Moneyway’. Plant Cell Tiss. Org. Cult. 37: 73-77CrossRefGoogle Scholar
  12. Zimmerman RH, Bhardwaj SV & Fordham IM (1995) Use of starch-gelled medium for tissue culture of some fruit crops. Plant Cell Tiss. Org. Cult. 43: 207-213Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Andrea Kodym
    • 1
  • Francisco Javier Zapata-Arias
    • 2
  1. 1.FAO/IAEA Agriculture and Biotechnology Laboratory, International Atomic Energy Agency LaboratoriesSeibersdorfAustria
  2. 2.FAO/IAEA Agriculture and Biotechnology Laboratory, International Atomic Energy Agency LaboratoriesSeibersdorfAustria

Personalised recommendations