Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 63, Issue 3, pp 207–214 | Cite as

Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation

  • J.P. Majada
  • F. Tadeo
  • M.A. Fal
  • R. Sánchez-Tamés
Article

Abstract

Dianthus caryophyllus cv. Nelken was cultured in vitro under different ventilation rates (0.11, 0.21, 0.68 and 0.86 changes h−1). Ventilation modified the anatomical characteristics of shoots and leaves described for plants grown in non-ventilated vessels: the cuticle became thicker, there was a decreased cell size and intracellular space size. Also, there were more photosynthetic and supportive tissues, including thicker cell walls. Increased ventilation promoted in vitro hardening of micropropagated carnation shoots, and pushed the culture-induced phenotype closer to that of ex vitro acclimatized plants. Anatomical variability of in vitro-grown plants was demonstrated to be a consequence of ventilation.

culture-induced-phenotype (CIP) Dianthus caryophyllus ex vitro acclimatization in vitro hardening 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brainerd KE & Fuchigami LH (1981) Acclimatization of aseptically cultured apple plants to low relative humidity. J. Am. Soc. Hort. Sci. 106: 515–518Google Scholar
  2. Brainerd KE, Fuchigami LH, Kwaitkowski S & Clark C S (1981) Leaf anatomy and water stress of aseptically cultured 'Pixy' plum grown under different environments. Hort. Sci. 16 (2): 173–175Google Scholar
  3. Capellades M, Fontarnau R, Carulla C & Debergh P (1990) Environment influences anatomy of stomata and epidermal cells in tissue-cultured in vitro and in vivo. J. Am. Soc. Hort. Sci. 115: 141–145Google Scholar
  4. Debergh P, Aitken-Christie J, Cohen D, Grout B, von Arnold S, Zimmerman R & Ziv M (1992) Reconsideration of the term 'hyperhydration' as used in micropropagation. Plant Cell Tiss. Org. Cult. 30: 135–140CrossRefGoogle Scholar
  5. Donnelly DJ & Vidaver WE (1984) Leaf anatomy of red raspberry transferred from in vitro culture to soil. J. Am. Soc. Hort. Sci. 109: 172–176Google Scholar
  6. Donnelly DJ, Vidaver WE & Lee KY (1985) The anatomy of tissue culture red raspberry prior to and after transfer to soil. Plant Cell Tiss. Org. Cult. 4: 43–50CrossRefGoogle Scholar
  7. Drew AP, Kavanagh KL & Maynard CA (1992) Acclimatizing micropropagated black cherry by comparison with half-sib seedlings. Physiol. Plant. 86: 459–464CrossRefGoogle Scholar
  8. Fabbri A, Sutter E & Dunston SK (1986) Anatomical changes in persistent leaves of tissue cultured strawberry plants after removal from culture. Sci. Hort. 28: 331–337CrossRefGoogle Scholar
  9. Fahn A (1982). Plant Anatomy. Elsevier Science Inc. New YorkGoogle Scholar
  10. Feder N & O'Brien TP (1968) Plant microtechnique: some principles and new methods. Am. J. Bot. 55: 123–127CrossRefGoogle Scholar
  11. Fisher BB (1968) Protein staining of ribboned epon sections for light microscopy. Histochemie 16: 92–96PubMedCrossRefGoogle Scholar
  12. Fujiwara K & Kozai T (1995) Physical microenvironment and its effects. In: Aitken-Christie A, Kozai T & Smith MAL (eds) Automation and Environmental Control in Plant Tissue Culture (pp. 301–318). Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar
  13. Goldstein JI, Newbury DE, Echlin P, Joy DC & Lifshin E (1992) Scanning Electron Microscopy and X-ray Microanalysis. Plenum Press, New YorkGoogle Scholar
  14. Grout BWW & Aston H (1978) Modified leaf anatomy of cauli-flower plantlets regenerated from meristem culture. Ann. Bot. 42: 993–995Google Scholar
  15. Kozai T, Fujiwara K & Hayashi M (1986) Fundamental studies of environments in plant tissue culture vessels II. Effects of stoppers and vessels on gas exchange rates between inside and outside of vessels closed with stoppers. J. Agric. Metereol. 42: 119–127Google Scholar
  16. Kozai T & Smith MAL (1995) Environmental control in plant tissue culture. In: Aitken-Christie A, Kozai T & Smith MAL (eds) Automation and Environmental Control in Plant Tissue Culture (pp. 301–318). Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar
  17. Lee N, Wetzsein HY & Sommer SE (1988) Quantum flux density effects on the anatomy and surface morphology of in vitro and in vitro-developed sweetgum leaves. J. Am. Soc. Hort. Sci 113: 167–171Google Scholar
  18. Majada JP (1995) Environmental control and automation of vitroponic cultures. PhD Thesis. Oviedo University, Oviedo, SpainGoogle Scholar
  19. Majada JP, Fal MA & Sanchez-Tamés R (1997) The effect of ventilation rate on proliferation and hyperhydricity of Dianthus caryophyllus L. In vitro Cell Dev. Biol. 33: 62–69Google Scholar
  20. Marin JA, Gella R & Herrero M(1988) Stomatal structure and functioning as a response to environmental changes in acclimatized micropropagated Prunus cerasus L. Ann. Bot 62: 663–670Google Scholar
  21. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  22. Nobel PS (1991) Physiochemical & Environmental Plant Physiology. Academic Press, New YorkGoogle Scholar
  23. Oppenheimer HR (1960) Adaptation to drought: Xerophytism plant-water relationships in arid and semiarid conditions. Rev. Res. Unesco: Arid. Zone Res. 15: 105–138Google Scholar
  24. Passioura JB (1982) Water in the soil-plant-atmosphere continuum. In: Lange OL, Nobel PS, Osmon, CB & Ziegler H (eds) Physiological Plant Ecology II. Encyclopedia of Plant Physiology, New Series, Vol 12B (pp. 5–33). Springer Verlag, BerlinGoogle Scholar
  25. Preece JE & Sutter EG (1991) Acclimatization of micropropagated plants to the greenhouse and field In: Debergh PC & Zimmermam RH (eds) Micropropagation: Technology and Application (pp. 77–93). Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar
  26. Shields LM (1950) Leaf xeromorphy as related to physiological and structural influences. Bot. Rev. 16: 399–447Google Scholar
  27. Smith MAL, Palta JP & McCown BH (1986) Comparative anatomy and physiology of microcultured seedling and greenhouse-grown in vitro. Can. J. Bot 62: 74–77Google Scholar
  28. Talon M, Tadeo FR & Zeevaart JAD (1991) Cellular changes induced by exogenous and endogenous gibberellins in shoot tips of long-day plant Silene armeria. Planta 185: 487–493CrossRefGoogle Scholar
  29. Vieitez AM, Ballester A, San-Jose MC & Vieitez E (1985) Anatomical and chemical studies of vitrified shoots of chestnut regenerated in vitro. Physiol. Plant. 65: 177–184CrossRefGoogle Scholar
  30. Werker E & Leshem B (1987) Structural changes during hyperhydration of carnation plantlets. Ann. Bot. 59: 377–385Google Scholar
  31. Wetzstein HY & Sommer HE (1982) Leaf anatomy of tissue cultured Liquidambar styraciflua (Hamamelidaceae) during acclimatization. Amer. J. Bot. 69: 1579–1586CrossRefGoogle Scholar
  32. Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants In: Debergh PC & Zimmermam RH (eds) Micropropagation Technology and Application (pp. 45–69). Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • J.P. Majada
    • 1
    • 1
  • F. Tadeo
    • 2
  • M.A. Fal
    • 3
  • R. Sánchez-Tamés
    • 3
  1. 1.Dpto. B.O.S. C/ Catedrático Rodrigo Uría, s/nLab. Fisiología VegetalOviedo, AsturiasSpain
  2. 2.Spain
  3. 3.Spain

Personalised recommendations