Breast Cancer Research and Treatment

, Volume 66, Issue 3, pp 249–254

CK-19 Expression by RT-PCR in the Peripheral Blood of Breast Cancer Patients Correlates with Response to Chemotherapy

  • Ana Rita Manhani
  • Reinaldo Manhani
  • Heloisa P. Soares
  • Israel Bendit
  • Fabiana Lopes
  • André G.B. Nicoletti
  • Fernando L.A. Fonseca
  • Mafalda Novaes
  • Simone M. Zatta
  • Vitor Arias
  • Sergioi Giralt
  • Auro del Giglio
Conference Report

DOI: 10.1023/A:1010621901102

Cite this article as:
Manhani, A.R., Manhani, R., Soares, H.P. et al. Breast Cancer Res Treat (2001) 66: 249. doi:10.1023/A:1010621901102

Abstract

Background. The recent introduction of sensitive RT-PCR-based techniques for the detection of epithelial antigen expression, such as CK-19, in the peripheral blood and bone marrow of breast cancer patients may provide an opportunity to evaluate tumor response at the molecular level, even in the absence of measurable disease while patients are still receiving chemotherapy.

Methods. We studied serially collected blood samples of 53 patients with breast cancer before, during, and after adjuvant, neoadjuvant, and palliative chemotherapy to evaluate its effects on the expression of CK-19 measured by RT-PCR.

Results. The percentage of CK-19 RT-PCR positivity decreased consistently from 43% (23/53) before chemotherapy to 14.3% (7/49), and to 18.9% (7/37) after 3 and 6 cycles, respectively (chi-square for linear trend=7.948; p=0.0048). Furthermore, there was a significant correlation between a negative CK-19 at three months and the response to chemotherapy (p=0.024).

Conclusion. We conclude that RT-PCR negativity for CK-19 expression at 3 months after the beginning of chemotherapy correlates with tumor response and, as treatment progresses, there is a significant trend for the occurrence of more negative RT-PCR results. Further studies are needed to confirm if this technique can be useful to assess response to chemotherapy in patients without measurable disease and if negativation of CK-19 expression while on chemotherapy is of prognostic significance.

breast neoplasms keratin/analysis prognosis prospective studies recurrence tumor markers 

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Ana Rita Manhani
    • 1
  • Reinaldo Manhani
    • 2
  • Heloisa P. Soares
    • 1
  • Israel Bendit
    • 2
  • Fabiana Lopes
    • 1
  • André G.B. Nicoletti
    • 1
  • Fernando L.A. Fonseca
    • 1
  • Mafalda Novaes
    • 1
  • Simone M. Zatta
    • 1
  • Vitor Arias
    • 3
  • Sergioi Giralt
    • 4
  • Auro del Giglio
    • 1
  1. 1.Disciplina de Hematologia e Oncologia da Faculdade de Medicina da Fundação ABCSão PauloBrazil
  2. 2.Fundação Pró-Sangue Hemocentro de São PauloSão PauloBrazil
  3. 3.Instituto Adolfo LutzSão PauloBrazil
  4. 4.MD Anderson Cancer CenterUniversity of TexasHoustonUSA

Personalised recommendations