Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 66, Issue 1, pp 31–34 | Cite as

Analysis of volatile compounds released from embryogenic cultures and somatic embryos of sweet oranges by head space SPME

  • G. Alonzo
  • F. Saiano
  • N. Tusa
  • S. Fatta Del BoscoEmail author
Article

Abstract

Volatile compounds released from callus and nucellar embryo tissues of ‘Valencia late’ and ‘Washington Navel’ sweet oranges (Citrus sinensis, L. Osbeck) were collected/concentrated by head space solid phase micro extraction and analysed by gas chromatography-mass spectrometry. Friable, white embryogenic cultures released a number of volatile compounds, including some essential oils. Different samples of the same embryogenic culture showed variability, possibly related to the presence of tissues undergoing differentiation. Analyses of the somatic embryos permitted the identification of several components, including limonene and methyl anthranilate. Considering the simplicity and the very small sample required (0.3 g of fresh tissue) head space solid phase micro extraction is suitable for studies and comparisons of volatile metabolites released from in vitro Citrus tissue cultures suggesting its potential in Citrus biochemical, genetic and breeding research.

Citrus sinensis essential oils GC-MS in vitro cultures 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alonzo G, Fatta Del Bosco S, Palazzolo E, Saiano F & Tusa N (2000a) Citrus cybrid leaf essential oil. Flav. Fragr. J. 15: 91-95CrossRefGoogle Scholar
  2. Alonzo G, Fatta Del Bosco S, Palazzolo E, Saiano F & Tusa N (2000b) Citrus somatic hybrid leaf essential oil. Flav. Frag. J. 15: 258-262CrossRefGoogle Scholar
  3. Mondello L, Dugo P, Basile A, Dugo G & Bartle K D (1995) Interactive use of linear retention indices, on polar and apolar columns, with MS library for reliable identification of complex mixtures. J. Microcol. Sep. 7: 581CrossRefGoogle Scholar
  4. Murashige T & Tucker DPH (1969) Growth factor requirements of citrus tissue culture. In: Proc. First Intl. Citrus Symp. 3: 1155-1161. University of CaliforniaGoogle Scholar
  5. Niedz RP, Moshonas Mg, Peterson B, Shapiro JP & Shaw PE (1997) Analysis of sweet orange (Citrus sinensis (L.) Osbeck) callus cultures for volatile compounds by gas chromatography with mass selective detector. Plant Cell Tiss. Org. Cult. 51: 181-185CrossRefGoogle Scholar
  6. Pawliszyn J (1997) Solid Phase Microextraction, theory and practice. Wiley-VCH Inc, USAGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • G. Alonzo
    • 1
  • F. Saiano
    • 1
  • N. Tusa
    • 2
  • S. Fatta Del Bosco
    • 3
    Email author
  1. 1.Dipartimento I.T.A.F., Sezione ChimicaUniversità di PalermoPalermoItaly
  2. 2.Istituto di Ricerca per la Genetica degli Agrumi, CNRPalermoItaly
  3. 3.Istituto di Ricerca per la Genetica degli Agrumi, CNRPalermoItaly

Personalised recommendations