Biotechnology Letters

, Volume 23, Issue 15, pp 1235–1241 | Cite as

Development of engineered biofilms on poly- L-lysine patterned surfaces

  • Stacie E. Cowan
  • Dorian Liepmann
  • Jay D. KeaslingEmail author


A technique has been developed to selectively attach bacteria to solid supports using poly-l-lysine. The patterned biofilms were labeled with green fluorescent protein (GFP) or a nucleic acid stain and imaged using both confocal microscopy and GFP stereomicroscopy. E. coli DH10B, E. coli MC1061, and Pseudomonas sp. GJ1 were selectively attached to regions coated with poly-l-lysine but not to uncoated regions. In contrast, E. coli DH5α, W3110 and 33456 attached indiscriminately to the coated and uncoated regions of the surface. Those organisms that selectively attached to the poly-l-lysine coated regions formed biofilms twice as thick as the organisms that attached indiscriminately to the surface. This technique can be used for selectively patterning surfaces with genetically engineered microorganisms for biosynthesis of secondary metabolites and biodegradation or for developing a bacterial-based microscale medical diagnostic tool.

E. coli poly-l-lysine selective attachment surface patterning 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Stacie E. Cowan
    • 1
  • Dorian Liepmann
    • 1
  • Jay D. Keasling
    • 1
    Email author
  1. 1.Joint Bioengineering Graduate ProgramUniversity of CaliforniaBerkeley and San FranciscoUSA

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