Biotechnology Letters

, Volume 23, Issue 14, pp 1165–1169 | Cite as

Kinetic model of lysozyme renaturation with the molecular chaperone GroEL

  • Xiao-Yan Dong
  • Yu-Bing Wang
  • Xiao-Guang Liu
  • Yan SunEmail author


From the renaturation kinetics of denatured/reduced lysozyme assisted by the molecular chaperone GroEL, a simplified kinetic model was established based on the competition between protein folding and aggregation. In the presence of GroEL and ATP, the aggregate formation was a second order reaction. With 2 mM ATP, a renaturation yield of 90% at a high renaturation rate was obtained when the molar ratio of GroEL to lysozyme was 1:1.

GroEL kinetics lysozyme model renaturation 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Xiao-Yan Dong
    • 1
  • Yu-Bing Wang
    • 2
  • Xiao-Guang Liu
    • 3
  • Yan Sun
    • 3
    Email author
  1. 1.Department of Biochemical EngineeringTianjin UniversityTianjinPeople's Republic of China
  2. 2.The University LibraryTianjin UniversityTianjinPeople's Republic of China
  3. 3.Department of Biochemical EngineeringTianjin UniversityTianjinPeople's Republic of China

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