Biotechnology Letters

, Volume 23, Issue 10, pp 761–766

Phytase production by high cell density culture of recombinant Bacillus subtilis

  • Antti Vuolanto
  • Niklas von Weymarn
  • Janne Kerovuo
  • Heikki Ojamo
  • Matti Leisola
Article

Abstract

Bacillus subtilis BD170, harboring a plasmid pGT44[phyC] carrying the phytase gene (phyC) and a phosphate-depletion inducible pst-promoter, was grown in a 2 l bioreactor. Using a controlled feeding of glucose, high cell densities of 32 and 56 g dry cell weight l−1 were achieved with peptone and yeast extract, respectively, as the complex nitrogen sources in a semi-defined growth medium. The fed-batch protocol was applied to production of recombinant phytase and a high extracellular phytase activity (48 U ml−1) was reached with peptone. Although the yeast extract feeding resulted in a higher cell density, it was unsuitable as a medium component for phytase expression due to its relatively high phosphate content.

Bacillus subtilis high cell density cultivation recombinant phytase 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Antti Vuolanto
    • 1
  • Niklas von Weymarn
    • 1
  • Janne Kerovuo
    • 2
  • Heikki Ojamo
    • 2
  • Matti Leisola
    • 1
  1. 1.Laboratory of Bioprocess EngineeringHelsinki University of TechnologyEspooFinland
  2. 2.Danisco Sugar & Sweeteners DevelopmentKantvikFinland

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