Biotechnology Letters

, Volume 23, Issue 10, pp 741–751 | Cite as

Ex vivo expansion of hematopoietic stem cells in bioreactors

  • J.M.S. Cabral


The in vitro culture of human hematopoietic stem cells in different types of bioreactors is reviewed. After a brief description of the hematopoiesis and stem cells biology, the selection of growth medium for stem cells is evaluated as well as the parameters to be controlled for ex vivo expansion and differentiation of stem cells. Several types of bioreactors for in vitro stem cell culture are discussed and compared.

differentiation expansion hollow fiber reactors packed bed reactors perfusion reactors stem cells stirred reactors 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • J.M.S. Cabral
    • 1
  1. 1.Centro de Engenharia Biológica e QuímicaInstituto Superior TécnicoLisboaPortugal

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