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Biotechnology Letters

, Volume 23, Issue 8, pp 575–582 | Cite as

A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors

  • Yeon Ho Je
  • Jin Hee Chang
  • Jae Young Choi
  • Jong Yul Roh
  • Byung Rae Jin
  • David R. O'Reilly
  • Seok Kwon Kang
Article

Abstract

A novel baculovirus genome has been generated that can be maintained in Escherichia coli and which facilitates the rapid and efficient generation of recombinant baculovirus expression vectors. This genome, designated bAcGOZA, lacks a portion of the essential ORF1629 gene but includes a miniF replicon and selectable kanamycin-resistance marker. The bAcGOZA can replicate only in E. coli and can be rescued by recombination with a transfer vector containing an intact ORF1629. There is no background of non-recombinant virus.

AcMNPV bacmid baculovirus expression vector miniF replicon 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Yeon Ho Je
    • 1
  • Jin Hee Chang
    • 1
  • Jae Young Choi
    • 1
  • Jong Yul Roh
    • 1
  • Byung Rae Jin
    • 2
  • David R. O'Reilly
    • 3
  • Seok Kwon Kang
    • 1
  1. 1.School of Agricultural BiotechnologySeoul National UniversitySuwonKorea
  2. 2.College of Natural Resources and Life ScienceDong-A UniversityPusanKorea
  3. 3.Department of Biology, Imperial College of Science, Technology and MedicineLondonUK

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