Behavior Genetics

, Volume 31, Issue 1, pp 79–91

Further Characterization and High-Resolution Mapping of Quantitative Trait Loci for Ethanol-Induced Locomotor Activity

  • Kristin Demarest
  • Jay Koyner
  • James McCaughranJr.
  • Laura Cipp
  • Robert Hitzemann
Article

Abstract

Differential sensitivity to the stimulant effects of ethanol on locomotor activity is determined in part by genetic differences. Among inbred strains of mice, moderate doses of ethanol (1-2 g/kg) stimulate locomotor activity in some strains, e.g., the DBA/2J (D2), but only mildly affect activity in other strains, e.g., C57BL/6J (B6) (Crabbe et al., 1982, 1983; Crabbe, 1986; Dudek and Phillips, 1990; Dudek et al., 1991; Dudek and Tritto, 1994). Quantitative trait loci (QTL) for the acute ethanol (1.5 g/kg) locomotor response has been identified in the BXD recombinant inbred (RI) series (N = 25 strains), a C57BL/6J × DBA/2J (B6D2) F2 intercross (N = 1800), and heterogeneous stock (HS) mice (N = 550). QTLs detected (p < .01) in the RI series were found on chromosomes 1, 2, and 6 and these QTLs were expressed in a time-dependent fashion. The QTLs on chromosomes 1 and 2 were confirmed in the F2 intercross at p < 10−7 or better. HS mice from G32 to G35 were used to fine-map the chromosome 2 QTL. Compared to the consensus map, the genetic map in the HS animals was expanded 10- to 15-fold. Over the region flanked by D2Mit94 to D2Mit304, three separate QTLs were detected in the HS animals. The data obtained confirm the usefulness of HS mice for the fine-mapping of QTLs to a resolution of 2 cM or less.

QTL mouse ethanol activity recombinant inbred fine-mapping 

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Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • Kristin Demarest
    • 1
  • Jay Koyner
    • 2
  • James McCaughranJr.
    • 2
  • Laura Cipp
    • 2
  • Robert Hitzemann
    • 2
    • 3
  1. 1.Department of Neurobiology and PsychiatryStony Brook
  2. 2.Department of PsychiatryStony Brook
  3. 3.Research and Psychiatry ServicesVeterans Administration Medical CenterNorthport

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