Advertisement

Applied Biochemistry and Microbiology

, Volume 37, Issue 3, pp 260–266 | Cite as

Isolation of Components of the Peptide Antibiotic Virginiamycin and Breeding of Their Producer, Streptomyces virginiae

  • V. I. Zvenigorodskii
  • B. V. Tyaglov
  • T. A. Voeikova
Article

Abstract

A method for chromatographic separation and quantitative determination of individual components of the antibiotic virginiamycin, produced by microbiological synthesis (Streptomyces virginiaestrain 147), is described. The components, M1–2and S1–5, were isolated from fermentation broth and identified by HPTLC and HPLC (the results obtained using the two methods correlate well with each other). The conditions of culturing of the producer and the composition of nutritive media were optimized. Using UV irradiation as a mutagenic factor, the producer was selected for increased level of synthesis of the antibiotic; this was achieved by inducing mutations that impart resistance to virginiamycin and meta-fluorophenylalanine, an analog of phenylalanine.

Keywords

Peptide Fermentation HPLC Streptomyces Phenylalanine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. 1.
    Cocito, C.G., Microbiol. Rev.1979, vol. 43, pp. 145-198.Google Scholar
  2. 2.
    Boon, B. and Devart, R., Analyst1974, vol. 99, no. 1, pp. 10-27.Google Scholar
  3. 3.
    Cocito, C.G., AntibioticsCorcoran, J.W. and Hahn, F.E., Eds., Berlin: Springer, 1983, pp. 296-332.Google Scholar
  4. 4.
    Kudryavtseva, E.A. and Voeikova, T.A., Biotekhnologiya, 1997, no. 6, pp. 3-18.Google Scholar
  5. 5.
    Di Giambattista, C.G. and Cocito C.G J. Antimicrob. Chemother., 1989, vol. 24, no. 3, pp. 485-507.Google Scholar
  6. 6.
    Biot, A.M., Biotechnology of Industrial Antibiotics, Vandamme, E.J., Ed., New York-Basel: Dekker, 1984, pp. 695-720.Google Scholar
  7. 7.
    Kingston, D.G.I., Kolpak, M.X., LeFevre, J.W., and Borup-Grochtman, I., J. Am. Chem. Soc., 1983, vol. 105, no. 15, pp. 5106-5110.Google Scholar
  8. 8.
    Molinero, A.A., Kingston, D.G.I., and Reed, J.W., J. Natural Products, 1989, vol. 52, no. 1, pp. 99-108.Google Scholar
  9. 9.
    Van Dijck, P.J. and Van Braekel, G., Chemotherapy, 1969, vol. 11, no. 1, pp. 109-114.Google Scholar
  10. 10.
    Zvenigorodsky, V.I., Emel'yanova, L.K., and Voeikova, T.A., Biotekhnologiya, 2000, no. 2., p. 7-13.Google Scholar
  11. 11.
    Tyaglov, B.V., Zvenigorodsky, V.I., Sisova, I.A., Polanuer, B.M., and Zhdanov, V.G., J. Planar Chromat., 1995, vol. 8, no. 4, pp. 374-377.Google Scholar
  12. 12.
    Degtiar, V.G., Tyaglov, B.V., and Degterev, E.V., J. Planar Chromat., 1994, vol. 7, no. 1, pp. 35-39.Google Scholar
  13. 13.
    Tyaglov, B.V., Zvenigorodsky, V.I., and Sizova, I.A., J. Planar Chromat., 1997, vol. 10, no. 3, pp. 200-203.Google Scholar
  14. 14.
    Demain, A.L., Biotechnology of Industrial AntibioticsVandamme, E.J., Ed., New York-Basel: Dekker, 1984, pp. 33-47.Google Scholar
  15. 15.
    Zvenigorodsky, V.I., Bugaichuk, Yu.D., Kuzovnikova, T.A., Lukin, A.A., and Zhdanov, V.G., Antibiotiki, 1981, no. 9, pp. 647-651.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • V. I. Zvenigorodskii
  • B. V. Tyaglov
  • T. A. Voeikova

There are no affiliations available

Personalised recommendations