Biochemistry (Moscow)

, Volume 66, Issue 7, pp 774–779

Laccases from Basidiomycetes: Physicochemical Characteristics and Substrate Specificity towards Methoxyphenolic Compounds

  • S. A. Smirnov
  • O. V. Koroleva
  • V. P. Gavrilova
  • A. B. Belova
  • N. L. Klyachko
Article

DOI: 10.1023/A:1010216829856

Cite this article as:
Smirnov, S.A., Koroleva, O.V., Gavrilova, V.P. et al. Biochemistry (Moscow) (2001) 66: 774. doi:10.1023/A:1010216829856

Abstract

Laccases from the Basidiomycetes Coriolus hirsutus, Coriolus zonatus, Cerrena maxima, and Coriolisimus fulvocinerea have been isolated and purified to homogeneity and partially characterized. The kinetics of oxidation of different methoxyphenolic compounds by the fungal laccases has been studied. As laccase substrates, such methoxyphenolic compounds as 4-hydroxy-3,5-dimethoxycinnamic acid (sinapinic acid), 4-hydroxy-3-methoxycinnamic acid (ferulic acid), and 2-methoxyphenol (guaiacol) were used. The stoichiometries of the enzymatic reactions were determined: guaiacol and sinapinic acid are one-electron donors and their oxidation apparently results in the formation of dimers. It was established that kcat/Km, which indicates the effectiveness of catalysis, increases in the series guaiacol, ferulic acid, and sinapinic acid. This fact might be connected with the influence of substituents of the phenolic ring of the substrates. This phenomenon was established for fungal laccases with different physicochemical properties, amino acid composition, and carbohydrate content. This suggests that all fungal laccases possess the same mechanism of interaction between organic substrate electron donors and the copper-containing active site of the enzyme and that this interaction determines the observed values of the kinetic parameters.

laccase methoxyphenolic compounds ferulic acid sinapinic acid guaiacol catalytic efficiency 

Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • S. A. Smirnov
    • 1
  • O. V. Koroleva
    • 2
  • V. P. Gavrilova
    • 3
  • A. B. Belova
    • 1
  • N. L. Klyachko
    • 1
  1. 1.School of ChemistryLomonosov Moscow State UniversityMoscowRussia
  2. 2.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  3. 3.Komarov Institute of BotanyRussian Academy of SciencesSt. PetersburgRussia

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