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Brief History of the Tissue Bank, Charles University Hospital, Hradec Králové, Czech Republic

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Abstract

A brief history of the Tissue Bank (TB) of the University Hospital Hradec Králové, Czech Republic, established by Dr. R. Klen in 1952 is presented. In Dr. Klen's original concept the TB was defined as a department specialised in the harvesting, processing, preservation, storage and distribution of various kinds of tissue for clinical and experimental practice. The first kinds of tissue collected in cadaveric donors were corneas, bone and skin. Xenogeneic cartilage and bone grafts were prepared at the same time. Later, preparation of soft connective tissues and chorion–amnion was introduced. During the first 15 years of activity a total of 11,443 grafts preserved by hypothermy at +4°C or freezing in absence of cryoprotectants (−20°C) were prepared. In the 60's freeze-drying of tissue grafts was introduced and the bank of cryopreserved cell lines was established. In the 80's cryopreservation of haematopoietic progenitor cells for clinical transplantations was started and the spectrum of tissue grafts was enlarged (xenogeneic pericardium and allogeneic specially treated dura mater for neurosurgical operations, pigskin for burn treatment, demineralised bone for parodontology and implantology). In the 90's human keratinocyte culture for treatment of burns and chronic skin defects was started. The human milk bank and organ bank co-operating with the Regional Transplantation Centre are component parts of the TB as well. The TB is an institutional member of the European Association of Tissue Banks and annually delivers approximately 1000 grafts that are used in University and county hospitals as well as in surgeons' private practices. Health insurance companies reimburse all grafts on a non-profit and tax-free basis.

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References cited

  • Bláha M et al. (1990) Transplantation of haematopoietic stem cells of peripheral blood-first experience (in Czech). Voj zdrav listy LIX: 218-223

    Google Scholar 

  • Dlabalová H et al. (1972) Treatment of leg ulcers with chorion grafts (In Czech). Čs Dermatol 47: 42

    Google Scholar 

  • Dostál F (1954) Proposal of the law on activities of tissue centres. In: Group of Authors. Tissue Transplantation (in Czech), pp 92-95. Státní zdravotnické nakladatelství. Praha

  • Group of authors (1954) Tissue Transplantation (in Czech). Státní zdravotnickénakladatelství, Praha

  • Heger J et al. (1971) Open defension of work of the collection of defined cells attached to the Tissue Bank of the University Hospital Hradec Králové (in Czech). Sborník v?d. prací LFKU v Hradci Králové, Suppl. 14: 129-173

    Google Scholar 

  • Heger J (1973) Freeze-drying of tissue grafts for transplantation (in Czech). Ph. D. Thesis. Charles University School of Pharmacy, Hradec Králové

    Google Scholar 

  • Human Milk Banking/Les Banques de lait humain (1981) Proceedings of the Commission C-I Meeting, Hradec Králové, Czechoslovakia, International Institute of Refrigeration, Paris

    Google Scholar 

  • Klein L et al. (1995) Clinical experience with skin xenografts in burned patients. In: Maselis M and Gunn SWA (eds) The Management of Burns and Fire Disasters: Perspectives 2000 Proceedings of the Second International Conference on Burns and Fire Disasters, pp 343-345. Palermo Kluwer, Academic Publishers

  • Klein L et al. (1997) Biological skin covers in treatment of two cases of the Lyell's syndrome. Annals of Transplantation 2: 45-48

    Google Scholar 

  • Klen R (1952) Tisssue centre (TC) (in Czech). Čs nemocnice 20: 120-121

    Google Scholar 

  • Klen R and Škrovina B (1955) Solution for preservation of bone tissue and cartilage (in Czech). Rozhl Chir XXXIV: 483-486

    Google Scholar 

  • Klen R and Škrovina B (1955) Growth zone-new material for bone plasties (in Czech). Rozhl Chir XXXIV: 479-483

    Google Scholar 

  • Klen R (1966) Long term results with processed heterogeneous bone grafts from the tissue bank in Hradec Králové (in Czech). Rozhl Chir XLV: 471-478

    Google Scholar 

  • Klen R (1957) Biological basis of tissue preservation (in Czech). Státní zdravotnické nakladatelství, Praha

  • Klen R (1982) Biological principles of tissue banking. Pergamon press, Oxford, New York, Toronto, Sydney, Paris, Frankfurt

    Google Scholar 

  • Klen R et al. (1965) Use of the anterior chamber of the eye for selection and preservation of cornea. Am J Ophthalmol 60: 879-889

    Google Scholar 

  • Klen R and Heger J (1969) A contribution to degradation of skin and cornea preserved hemibiotically in the temperature near 0°C. Acta Chir Plast 11: 47-55

    Google Scholar 

  • Klen R (1959) Cold shock. Folia Biol 1/V: 82-83

    Google Scholar 

  • Klen R (1961) Entstehung von Metastasen bei Verschieden nach Entfernung von Bösartigen Knochenneubildungen verwendeten Knochenspänen. Radiobiol Radiother 2/1: 109-115

    Google Scholar 

  • Klen R et al. (1977) Freeze-dried-homogeneous graft of fascia lata in neurosurgery. J Neurosurg Sci 21: 247-250

    Google Scholar 

  • Klen R et al. (1968) Experimental study of factors influencing take and survival of human skin homografts. Plast Reconstr Surg 41: 471-476

    Google Scholar 

  • Klen R and Skalská H (1976) A comparison of dermoepidermal and chorionamniotic grafts in the treatment of burns. Acta Chir Plast 18: 225-232

    Google Scholar 

  • Klen R (1976) Tissue banking in Czechoslovakia. Transplantation Proceedings VIII, Suppl. 1: 49-51

  • Klen R (1981) Human milk bank at the Medical Faculty Hospital in Hradec Králové (aspects of organisation). In: Human Milk Banking /Les Banques de lait humain. Proceedings of the Commission C-I Meeting, Hradec Králové, Czechoslovakia, pp 19-23. International Institute of Refrigeration, Paris

    Google Scholar 

  • Mareček V et al. (1979) Long-term results of treatment using heteregenous grafts at the Department of Orthopaedic Surgery of the University Hospital in Hradec Králové (in Czech). Sborník v?d prací LFKU v Hradci Králové, Suppl.: 119-122

  • Měřička P and Klen R (1979) Short-term clinical results with application of long term stored freeze-dried bone and cartilage grafts (in Czech). Sborník v?d prací LFKU v Hradci Králové, Suppl.: 29-42

  • Měřička P (1982) Thirty Years of The Tissue Bank of the University Hospital Hradec Králové (in Czech). Léka?ské zprávy (Hradec Králové) 27: 195-206

    Google Scholar 

  • Měřička P et al. (1986) Vorbereitung und klinische Anwendung von xenogenen durch Glutaraldehyd fixierten lyophilisierten Pericardium Transplantaten. Probleme derHämatologie Transfusion und Transplantation 13: 345-358

    Google Scholar 

  • Měřička P (1991) Preservation and Clinical Application of Soft Tissue Grafts. Charité Report 7: 8

    Google Scholar 

  • Měřička P (1991) Specially treated freeze-dried dura mater-perspective material for duraplasties (in Czech). Léka?ské zprávy, Hradec Králové 36: 49-59

    Google Scholar 

  • Měřička P et al. (1987) Cryopreservation of dermoepidermal grafts for treatment of burns. In Proceedings of the XVIIth International Congress of Refrigeration, Commission C-I, Vienna 24-29.8. 1987, pp 1-8. International Institute of Refrigeration, Paris

    Google Scholar 

  • Měřička P et al. (1995) The role of the tissue bank in disaster planing. In Maselis M and Gunn SWA (eds) The Management of Burns and Fire Disasters: Perspectives 2000. Proceedings of the Second International Conference on Burns and Fire Disasters, pp 75-83. Kluwer Academic Publishers, Palermo

    Google Scholar 

  • Měřička P et al. (1988) Low Temperature preservation of collageneic tissues for transplantation purposes and their possible use. In Proceedings of the XVIIth International Congress of Refrigeration, Commission C-I, Vienna. In Proceedings of the XVIIth International Congress of Refrigeration, Commission C-I, pp 9-15. International Institute of Refrigeration, Paris

    Google Scholar 

  • Měřička P et al. (1991) Containers for freezing and storage of bone marrowstem cells. Sb. v?d. prací Lék. Fak. Karlovy Univ., Hradec Králové 34: 367-387

    Google Scholar 

  • Měřička P et al. (1997) Validation of the standard operating procedure for cryopreservation of peripheral blood progenitor cells (PBPC). In IIR International Conference, Commission C-I Cryoprotectants in Medical Practice May 12-15 1997, Hradec Králové, Long Abstracts, p 19 International Institute of Refrigeration, Paris

    Google Scholar 

  • Měřička P et al. (1998) Practical aspects of establishing an allogeneic keratinocyte bank. Roczniki Oparzen, Annals of Burns 105-109

  • Měřička P (1996) Tissue banking in the Czech Republic-current status. In Byk JRCh, Lechat A and von Versen R (eds) 4th International Conference European Association of Tissue Banks, pp 47-50. Monduzzi Editore, Bologna

    Google Scholar 

  • Měřička P et al. (1998) Biological skin cover: banking and application. In: Phillips GO, Strong DM, von Versen R and Nather A (eds) Advances in Tissue Banking, vol 2, pp 323-341. World Scientific Singapore, New Jersey, London, Hong Kong

    Google Scholar 

  • Moserová J et al. (1974) Bacteriological control of dermoepidermal grafts from porcine hides (in Czech) Rozhl Chir 53: 631-634

    Google Scholar 

  • Pařízek J et al. (1989) Xenogeneic pericardium as a dural substitute in reconstruction in suboccipital dura mater in children. J Neurosurg 70: 905-909

    Google Scholar 

  • Pařízek J and Měřička P (1990) Duraplasty with pre-treated freeze-dried sterilized human dura mater. Sborník v?d. prací Lék. Fak. Karlovy Univ. Hradec Králové 30: 135-143

    Google Scholar 

  • Rozsíval P and Měřička P (1988) Unsere Erfahrungen bei Operationen mit Skleraplastiken nach Pivovarow. Folia Ophthalmol 13: 121-123

    Google Scholar 

  • Rozsíval P et al. (1997) Long-term results of scleral reinforcement in children. In Süveges I and Folmann P (eds) SOE 97 1st edition, pp 957-961. Monduzzi edittore, Bologna

    Google Scholar 

  • Straková H and Měřička P (1991) Analysis of demand of grafts used for dura mater plasty in the Czech and Slovak Federal Republic (in Czech). Lék. zpr. LF UK, Hradec Králové 36: 135-143

    Google Scholar 

  • Straková H et al. (1995) Culture of epithelial Sheets from Cryopreserved keratinocyte primocultures. Journal of Experimental and Clinical Cancer Research 14 Suppl.: 99-101

    Google Scholar 

  • Svěrák J et al. (1979) Fascia lata in the Retina Detachment Surgery. Sborník v?d prací Lék. Fak. Karlovy Univ. Suppl.: 123-126

  • Šimünek A and Kopecká D (1998) Our experience with use of freeze-dried demineralised bone in dental implantology (in Czech). LKS (Chamber of Stomatologists), 8: 8-9

    Google Scholar 

  • Vávra L (1981) Treatment of Human Milk. In Human Milk Banking-Les Banques de lait humain. Proceedings of the C-1 Commission Meeting, Hradec Králové, pp 25-31. International Institute of Refrigeration, Paris

    Google Scholar 

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Authors and Affiliations

  1. University Hospital, 500 05, Hradec Králové, Czech Republic

    Pavel Měřička (Head of the Tissue Bank)

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  1. Pavel Měřička
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Měřička, P. Brief History of the Tissue Bank, Charles University Hospital, Hradec Králové, Czech Republic. Cell Tissue Banking 1, 17–25 (2000). https://doi.org/10.1023/A:1010128228619

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