Plant Ecology

, Volume 145, Issue 2, pp 351–358

Photoinhibition by visible and ultraviolet radiation in the red macroalga Porphyra umbilicalis grown in the laboratory

  • Donat-P. Häder
  • Almut Gröniger
  • Caroline Hallier
  • Michael Lebert
  • Felix L. Figueroa
  • Carlos Jiménez


Photosynthetic oxygen production and PAM fluorescence measurements were used to follow photoinhibition in the red macroalga Porphyra umbilicalis. Exposure to simulated solar radiation caused inhibition of the effective photosynthetic quantum yield from which the thalli partially recovered in the shade in subsequent hours. There were no significant differences between samples exposed to unfiltered radiation and those exposed to radiation from which increasing portions of UV radiation had been removed indicating that the thalli are well adapted to current levels of solar PAR and UV radiation. This notion was supported by the finding of high concentrations of UV screening pigments which were even enhanced by exposure to increased UV radiation. However, when exposed to (only) UV radiation about 50% higher than that encountered by the organisms in their natural habitat, the photosynthetic yield decreased slowly and did not show any recovery even when the degree of inhibition did not exceed 10%.

oxygen measurements PAM fluorescence photoinhibition Porphyra rhodophyta ultraviolet radiation 


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  1. Abe, S.-I., Murakami, A., Ohki, K., Aruga, Y. & Fujita, Y. 1994. Changes in stoichiometry among PSI, PSII and Cyt b6-f complexes in response to chromatic light for cell growth observed with the red alga Porphyra yezoensis. Plant Cell Physiol. 35: 901–906.Google Scholar
  2. Beach, K. S. & Smith, C. M. 1996a. Ecophysiology of tropical rhodophytes. I. Microscale acclimation in pigmentation. J. Phycol. 32: 701–710.Google Scholar
  3. Beach, K. S. & Smith, C. M. 1996b. Ecophysiology of tropical rhodophytes. II. Microscale acclimation in photosynthesis. J. Phycol. 32: 710–718.Google Scholar
  4. Björkman, O. & Demmig, B. 1987. Photon yield of O2-evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. Planta 170: 489–504.Google Scholar
  5. Büchel, C. & Wilhelm, C. 1993. In vivo analysis of slow chlorophyll fluorescence induction kinetics in algae: progress, problems and perspective. Photochem. Photobiol. 58: 137–148.Google Scholar
  6. Coll, J. & Cox, J. 1977. The genus Porphyra C. Ag. (Rhodophyta, Bangiales) in the American north Atlantic. I. New species from North Carolina. Bot. Marina 20: 155–159.Google Scholar
  7. Conway, E. 1964a. Autecological studies of the genus Porphyra. I. The species found in Britain. Brit. Phycol. Bull. 2: 342–348.Google Scholar
  8. Conway, E. 1964b. Autecological studies of the genus Porphyra. II. Porphyra umbilicalis. Brit. Phycol. Bull. 2: 349–363.Google Scholar
  9. Dring, M. J., Makarov, V., Schoshina, E., Lorenz, M. & Lüning, K. 1996. Influence of ultraviolet radiation on chlorophyll fluorescence and growth in different life history stages of three species of Laminaria (Phaeophyta). Marine Biol. 126: 183–191.Google Scholar
  10. Figueroa, F. L., Jiménez, C., Pérez-Lloréns, J. L. & Niell, F. X. (eds). 1996. Underwater light and algal photobiology. Scientia Marina 60 (Suppl. 1), Barcelona, Spain, p. 343.Google Scholar
  11. Figueroa, F. L., Salles, S., Aguilera, J., Jiménez, C., Mercado, J., Viñegla, B., Flores-Moya, A. & Altamirano, M. 1997. Effects of solar radiation on photoinhibition and pigmentation in the red alga Porphyra leucosticta. Mar. Ecol. Progr. Ser. 151: 81–90.Google Scholar
  12. Fork, D. C., Öquist, G. & Hoch, G. E. 1982. Fluorescence emission from photosystem I at room temperature in red alga Porphyra perforata. Plant Sci. Lett. 24: 249–254.Google Scholar
  13. Franklin, L. A. & Forster, R. 1997 The changing irradiance environment: consequences for marine macrophyte physiology, productivity and ecology. Eur. J. Phycol. 32: 207–232.Google Scholar
  14. Franklin, L. A., Levavasseur, G., Osmond C. B., Henley, W. J. & Ramus, J. 1992. Two components of onset and recovery during photoinhibition of Ulva rotundata. Planta 186: 399–408.Google Scholar
  15. Franklin, L. A., Seaton, G. G. R., Lovelock, C. E. & Larkum, A. W. D. 1996. Photoinhibition of photosynthesis on a coral reef. Plant, Cell Env. 19: 825–836.Google Scholar
  16. Genty, B. E., Briantais, J. M. & Baker, N. R. 1989. Relative quantum efficiencies of the two photosystems of leaves in photorespiratory and non-photorespiratory conditions. Plant Physiol. Biochem. 28: 1–10.Google Scholar
  17. Häder, D.-P. 1997. UV-B and aquatic ecosystems. Pp. 4–13. In: Rozema, J., Gieskes, W. W. C., van de Geijn, S. C., Nolan, C. & de Boois, H. (eds). UV-B and Biosphere, Kluwer Academic Publishers, Dordrecht.Google Scholar
  18. Häder, D.-P. & Figueroa, F. L. 1997. Photoecophysiology of marine macroalgae. Photochem. Photobiol. 66: 1–14.Google Scholar
  19. Häder, D.-P., Herrmann, H. & Santas, R. 1996a. Effects of solar radiation and solar radiation deprived of UV-B and total UV on photosynthetic oxygen production and pulse amplitude modulated fluorescence in the brown alga Padina pavonia. FEMS Microbiol. Ecol. 19: 53–61.Google Scholar
  20. Häder, D.-P., Herrmann, H., Schäfer, J. & Santas, R. 1996b. Photosynthetic fluorescence induction and oxygen production in corallinacean algae measured on site. Bot. Acta 109: 285–291.Google Scholar
  21. Häder, D.-P., Lebert, M., Flores-Moya, A., Jiménez, C., Mercado, J., Salles, S., Aguilera, J. & Figueroa, F. L. 1997b. Effects of solar radiation on the photosynthetic activity of the red alga Corallina elongata Ellis et Soland. J. Photochem. Photobiol. B: Biol. 37: 196–202.Google Scholar
  22. Häder, D.-P., Lebert, M., Mercado, J., Aguilera, J., Salles, S., Flores-Moya, A., Jiménez, C. & Figueroa, F. L. 1996c. Photosynthetic oxygen production and PAMfluorescence in the brown alga Padina pavonica (Linnaeus) Lamouroux measured in the field under solar radiation. Mar. Biol. 127: 61–66.Google Scholar
  23. Häder, D.-P., Porst, M., Herrmann, H., Schäfer, J. & Santas, R. 1996a. Photoinhibition in the Mediterranean green alga Halimeda tuna Ellis et Sol measured in situ. Photochem. Photobiol. 64: 428–434.Google Scholar
  24. Häder, D.-P., Porst, M., Herrmann, H., Schäfer, J. & Santas, R. 1997a. Photosynthesis of the Mediterranean green alga Caulerpa prolifera measured in the field under solar irradiation. J. Photochem. Photobiol. 37: 66–73.Google Scholar
  25. Häder, D.-P. & Schäfer, J. 1994a. Photosynthetic oxygen production in macroalgae and phytoplankton under solar irradiation. J. Plant Physiol. 144: 293–299.Google Scholar
  26. Häder, D.-P. & Schäfer, J. 1994b. In-situ measurement of photosynthetic oxygen production in the water column. Env. Monitoring Assessm. 32: 259–268.Google Scholar
  27. Hanelt, D., Hupperts, K. & Nultsch, W. 1992. Photoinhibition of photosynthesis and its recovery in red algae. Botanica Acta 105: 278–284.Google Scholar
  28. Hanelt, D., Hupperts, K. & Nultsch, W. 1993. Daily course of photosynthesis and photoinhibition in marine macroalgae investigated in the laboratory and field. Mar. Ecol. Progr. Ser. 97: 31–37.Google Scholar
  29. Helbling, E. W., Villafañe, V., Ferrario, M. & Holm-Hansen, O. 1992. Impact of natural ultraviolet radiation on rates of photosynthesis and on specific marine phytoplankton species. Mar. Ecol. Prog. Ser. 80: 89–100.Google Scholar
  30. Herbert, S. K. 1990. Photoinhibiton resistance in the red alga Porphyra perforata, Plant Physiol. 92: 514–519.Google Scholar
  31. Herrmann, H., Ghetti, F., Scheuerlein, R. & Häder, D.-P. 1995. Photosynthetic oxygen and fluorescence measurements in Ulva laetevirens affected by solar irradiation. J. Plant Physiol. 145: 221–227.Google Scholar
  32. Herrmann, H., Häder, D.-P., Köfferlein, M., Seidlitz, H. K. & Ghetti, F. 1996. Effects of UV radiation on photosynthesis of phytoplankton exposed to solar simulator light. J. Photochem. Photobiol. B: Biol. 3: 21–28.Google Scholar
  33. Karentz, D., McEuen, F. S., Land, M. C. & Dunlap, W. C. 1991. Survey of mycosporine-like amino acid compounds in Antarctic marine organisms: potential protection from ultraviolet exposure. Marine Biol. 108: 157–166.Google Scholar
  34. Kirst, G. O. & Wiencke, C. 1996. Ecophysiology of algae. J. Phycol. 31: 181–199.Google Scholar
  35. Larkum, A. W. D. & Wood, W. F. 1993. The effect of UV-B radiation on photosynthesis and respiration of phytoplankton, benthic macroalgae and seagrasses. Photosynth. Res. 36: 17–23.Google Scholar
  36. Leverenz, J. W., Falk, S., Pilström, C.-M. & Samuelsson, G. 1990. The effects of photoinhibition on the photosynthetic light-response curve of green plant cells (Chlamydomonas reinhardtii). Planta 182: 161–168.Google Scholar
  37. Lopez-Figueroa, F. 1992. Diurnal variation in pigment content in Porphyra laciniata and Chondrus crispus and its relation to the diurnal changes of underwater light quality and quantity. P. S. Z. N. I. Marine Ecol. 13: 285–305.Google Scholar
  38. Lüning, K. 1990. Seaweeds. Their Environment, Biogeography and Ecophysiology. Wiley, New York, p. 527.Google Scholar
  39. Markager, S. & Sand-Jensen, K. 1994. The physiology and ecology of light-grown relationship in macroalgae. Pp. 209–298. In: Round, F. E. & Chapman, D. J. (eds). Progress in Phycological Research, Vol. 10, Biopress Ltd., Bristol.Google Scholar
  40. Merrill, J. E., Mimuro, M., Aruga, Y. & Fujita, Y. 1983. Lightharvesting for photosynthesis in four strains of the red alga Porphyra yezoensis having different phycobilin contents. Plant Cell Physiol. 24: 261–266.Google Scholar
  41. Porst, M., Herrmann, H., Schäfer, J., Santas, R. & Häder, D.-P. 1997. Photoinhibition in the Mediterranean green alga Acetabularia mediterranea measured in the field under solar irradiation. J. Plant Phys. 151: 25–32.Google Scholar
  42. Schreiber, U., Bilger, W. & Neubauer, C. 1994. Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. Pp. 49–70. In: Schulze, E. D. & Caldwell, M. M. (eds). Ecophysiology of Photosynthesis, Ecological Studies, Vol. 100, Springer-Verlag, Berlin.Google Scholar
  43. Schreiber, U., Schliwa, U. & Bilger, W. 1986. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10: 51–62.Google Scholar
  44. Starr, R. & Zeikus, J. A. 1987. UTEX: The culture collection of algae at the university of Texas at Austin. J. Phycol. 23 (Suppl.): 1–47.Google Scholar
  45. Wiencke, C., Bartsch, I., Bischoff, B., Peters, A. F. & Breeman, A. M. 1994. Temperature requirements and biogeography of Antarctic, Arctic and amphiequatorial seaweeds. Bot. Marina 37: 247–259.Google Scholar
  46. Yoon, G. J., Lee, T. Y., O'Hara, E. P., Moore, T. A., Yoon, M. & Song, P.-S. 1981. The spectroscopy of Porphyra sp. in situ. Can. J. Spect. 26: 150–157.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Donat-P. Häder
    • 1
  • Almut Gröniger
    • 1
  • Caroline Hallier
    • 2
  • Michael Lebert
    • 1
  • Felix L. Figueroa
    • 3
  • Carlos Jiménez
    • 3
  1. 1.Institut für Botanik und Pharmazeutische BiologieFriedrich-Alexander-UniversitätErlangenGermany
  2. 2.School of Biological SciencesUniversity of PortsmouthPortsmouthGreat Britain
  3. 3.Departamento de Ecologia, Facultad de CienciasUniversidad de MálagaMálagaSpain

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