Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis
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Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.
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