Methods in Cell Science

, Volume 20, Issue 1–4, pp 71–78 | Cite as

Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis

  • David A. Mills
  • Trevor G. Phister
  • Kathleen A. Baldwin
  • Gary M. Dunny
  • Larry L. McKay
Article

Abstract

Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.

Conjugation oriT Mobilization Lactic acid bacteria Lactococci 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • David A. Mills
    • 1
  • Trevor G. Phister
    • 1
  • Kathleen A. Baldwin
    • 2
  • Gary M. Dunny
    • 1
  • Larry L. McKay
    • 2
  1. 1.Department of MicrobiologyUniversity of Minnesota
  2. 2.Department of Food Science and NutritionUniversity of MinnesotaSt PaulUSA

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