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Methods in Cell Science

, Volume 19, Issue 1, pp 25–31 | Cite as

Assessment of cyclooxygenase inhibitors using in vitro assay systems

  • Mei-Shiang Jang
  • John M. Pezzuto
Article

Abstract

Various inhibitors of cyclooxygenase are known to mediate cancer chemopreventive effects. We currently describe two in vitro assay systems for measuring cyclooxygenase activity. These assays can be used in combination and thereby provide a rapid, reliable, and economical approach that is applicable for large-scale evaluation of test samples. This approach employs peroxidase co-substrate oxidation and oxygen consumption assays. The former system, adapted to a 96-well plate format, detects inhibitors that function as a radical scavengers or interact with the enzyme directly. The latter system specifically monitors cyclooxygenase inhibitors that interact with the enzyme itself. Thus, the peroxidase co-substrate oxidation assay serves as a pre-screening method, whereas the oxygen consumption assay is used subsequently to investigate the mode of action mediated by samples which test positive.

Assay Co-oxidation Cyclooxygenase Inhibitor Oxygen consumption Peroxidase 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Mei-Shiang Jang
    • 1
  • John M. Pezzuto
    • 1
  1. 1.Program for Collaborative Research in Pharmaceutical Sciences, Department of Medicinal Chemistry and PharmacognosyUniversity of Illinois at ChicagoChicagoUSA

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