Molecular Breeding

, Volume 3, Issue 2, pp 105–113 | Cite as

RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population

  • Ning Huang
  • Arnold Parco
  • Teresita Mew
  • Gerard Magpantay
  • Susan McCouch
  • Emmanuel Guiderdoni
  • Jichen Xu
  • Prasanta Subudhi
  • Enrique R. Angeles
  • Gurdev S. Khush
Article

Abstract

We have developed an RFLP framework map with 146 RFLP markers based on a doubled haploid population derived from a cross between an indica variety IR64 and a japonica variety Azucena. The population carries 50.2% of IR64 loci and 49.8% of Azucena loci, indicating an equal amount of genetic materials from each parent has been transmitted to the progenies through anther culture. However, some markers show segregation distortion. These distorted marker loci are located on 10 chromosomal segments. Using this map we were able to place 8 isozymes, 14 RAPDs, 12 cloned genes, 1 gene for brown planthopper (BPH) resistance, and 12 QTLs for grain length, grain width and length/width ratio onto rice chromosomes. The major gene for BPH resistance was mapped on chromosome 12 near RG463 and isozyme Sdh-1. Most of the QTLs identified for the three grain characters were closely linked on chromosomes 1, 2, 3 and 10. We concluded that the RFLP framework map presented here will be useful for mapping other genes segregating in this doubled haploid population. Thus rapid generation of doubled haploid lines and their unbiased segregation make it very attractive for gene mapping.

DNA markers genetic linkage map marker-assisted breeding Oryza sativa 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Ning Huang
    • 1
  • Arnold Parco
    • 1
  • Teresita Mew
    • 1
  • Gerard Magpantay
    • 1
  • Susan McCouch
    • 2
  • Emmanuel Guiderdoni
    • 3
  • Jichen Xu
    • 1
  • Prasanta Subudhi
    • 1
  • Enrique R. Angeles
    • 1
  • Gurdev S. Khush
    • 1
  1. 1.International Rice Research InstituteManilaPhilippines
  2. 2.Department of Plant BreedingCornell UniversityIthacaUSA
  3. 3.CIRAD-CA 5035Montpellier Cedex 1France

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