Molecular Breeding

, Volume 6, Issue 2, pp 125–134 | Cite as

AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.)

  • I. Roldán-Ruiz
  • J. Dendauw
  • E. Van Bockstaele
  • A. Depicker
  • M. De Loose


An evaluation was performed of the potential use of AFLP markers to reveal polymorphisms among Lolium perenne plants with different degrees of kinship. Radioactive and fluorescent detection techniques were applied. The use of a fluorescent detection approach contributed greatly to the speed and ease of conducting and interpreting the AFLP patterns. The great discriminative power of AFLP markers and their capacity to represent genetic relationships among ryegrass plants was shown. Despite the high polymorphic value of the AFLP markers, standard statistical tests could not differentiate between two gene pools derived from different breeding programmes. It proved also impossible to correlate fodder and turf phenotypes with AFLP distance data. A very important point revealed by our data is the high degree of genetic diversity within commercial ryegrass varieties. Our findings are relevant to any outcrossing crop with a breeding strategy based on the production of synthetic populations.

ryegrass gene pool AFLP fingerprinting genetic distance genetic diversity 


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  1. 1.
    Baril CP, Verhaegen D, Vigneron P, Bouvet JM, Kremer A: Structure of the specific combining ability between two species of Eucalyptus. I. RAPD data. Theor Appl Genet 94: 796-803 (1997).Google Scholar
  2. 2.
    Becker J, Vos P, Kuiper M, Salamini F, Heun M: Combined mapping of AFLP and RFLP markers in barley. Mol Gen Genet 249: 65-73 (1995).Google Scholar
  3. 3.
    Bernardo R: Estimation of coefficient of coancestry using molecular markers in maize. Theor Appl Genet 85: 1055-1062 (1993).Google Scholar
  4. 4.
    Booy G, van Dreven F, Steverink-Raben A: Identification of ryegrass varieties (Lolium spp.) using allele frequencies of the PGI-2 and ACP-1 isozyme systems. Plant Var Seeds 6: 179-196 (1993).Google Scholar
  5. 5.
    Charmet G, Balfourier F: Isozyme variation and species relationships in the genus Lolium L. (ryegrasses, Gramineaceae). Theor Appl Genet 87: 641-649 (1994).Google Scholar
  6. 6.
    Dillmann C, Bar-Hen A, Guérin D, Charcosset A, Murigneux A: Comparison of RFLP andmorphological distances between maize Zea mays L. inbred lines. Consequences for germplasm protection purposes. Theor Appl Genet 95: 92-102 (1997).Google Scholar
  7. 7.
    Jaccard P: Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44: 223-270 (1908).Google Scholar
  8. 8.
    Hill MO: DECORANA: a Fortran program for detrended correspondence analysis and reciprocal averaging. Cornell University, Ithaca NY (1979).Google Scholar
  9. 9.
    Hongtrakul V, Huestis GM, Knapp SJ: Amplified fragment length polymorphisms as a tool for DNA fingerprinting sun-flower germplasm: genetic diversity among oilseed inbred lines. Theor Appl Genet 95: 400-407 (1997).Google Scholar
  10. 10.
    Lanza LLB, de Souza CLJ, Ottoboni LMM, Vieira MLC, de Souza AP: Genetic distance of inbred lines and prediction of maize single-cross performance using RAPD markers. Theor Appl Genet 94: 1023-1030 (1997).Google Scholar
  11. 11.
    Law JR, Donini P, Koebner RMD, Reeves JC, Cooke RJ: Statistical methods for assessing and interpreting genetic diversity. In: Advances in Biometrical Genetics. Proceedings of the 10th meeting of the Eucarpia Section, Biometrics in Plant Breeding, Poznan (1997).Google Scholar
  12. 12.
    Norusis NJ: SPSS for windows: base system. User's Guide, Release 6.0 (1993).Google Scholar
  13. 13.
    Paglia G, Morgante M: PCR-based multiplex DNA fingerprinting techniques for the analysis of conifer genomes. Mol Breed 4: 173-177 (1998).Google Scholar
  14. 14.
    Paran I, Aftergoot E, Shifriss C: Variation in Capsicum annuum revealed by RAPD and AFLP markers. Euphytica 99: 167-173 (1998).Google Scholar
  15. 15.
    Perkin Elmer, Applied Biosystems: ABI Prism GeneScan Analysis Software. User's manual (1996a).Google Scholar
  16. 16.
    Perkin Elmer, Applied Biosystems: ABI Prism Genotyper 2.0. User's manual, (1996).Google Scholar
  17. 17.
    Reamon-Buttner SM, Schoudelmaer J, Jung C: AFLP markers linked to the sex locus in Asparagus officinalis L. Mol Breed 4: 91-98 (1998).Google Scholar
  18. 18.
    Roldán-Ruiz I, Van Laecke K, De Riek J, Calsyn E, Depicker A, Van Bockstaele E, Beerepoot L., De Loose M: The use of molecular markers for assessing genetic conformity between varieties of ryegrass. Med Fac Landbouw Univ Gent. 62/4a (1997).Google Scholar
  19. 19.
    Roldán-Ruiz I, Depicker A, Dendauw J, Van Bockstale E, De Loose M: Comparison of different methods to score radioactive AFLP gels. Proceedings of the 6th Plant and Animal Genome Meeting, San Diego, CA (1998).Google Scholar
  20. 20.
    ter Braak CJF: CANOCO: a Fortran program for canonical community ordination by [partial] [detrended] [canonical] correspondence analysis, principal components analysis and redundancy analysis (version 2.1.). Agricultural Mathematics Group, Wageningen, Netherlands (1978).Google Scholar
  21. 21.
    Tsegaye S, Tesemma T, Belay G: Relationships among tetraploid wheat (Triticum turgidum L.) landrace populations revealed by isozyme markers and agronomic traits. Theor appl Genet 93: 600-605 (1996).Google Scholar
  22. 22.
    Van Bockstaele E: Aspects of breeding perennial ryegrass (Lolium perenne). Plant Sci 35: 690-694 (1998).Google Scholar
  23. 23.
    Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M: AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407-4414 (1995).Google Scholar
  24. 24.
    Weising K, Beyermann B, Ramser J, Kahl G: Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis 12: 159-169 (1991).Google Scholar
  25. 25.
    Zabeau M, Vos P: European Patent Application, publication EP 0534858 (1993).Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • I. Roldán-Ruiz
    • 1
  • J. Dendauw
    • 1
  • E. Van Bockstaele
    • 1
  • A. Depicker
    • 2
  • M. De Loose
    • 3
  1. 1.Department of Plant Genetics and BreedingCentre of Agricultural Research-GentMelleBelgium
  2. 2.Department of Genetics, Flanders Interuniversity Institute for BiotechnologyUniversity of GentGentBelgium
  3. 3.Department of Plant Genetics and BreedingCentre of Agricultural Research-GentMelleBelgium

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