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Molecular Breeding

, Volume 4, Issue 4, pp 313–319 | Cite as

Potato tubers as a biofactory for recombinant antibodies

  • Olga Artsaenko
  • Barbara Kettig
  • Ulrike Fiedler
  • Udo Conrad
  • Klaus Düring
Article

Abstract

Potato tubers have been successfully used for high-level production of a recombinant single-chain Fv (ScFv) antibody. Ubiquitous high-level expression was achieved under control of the CaMV 35S promoter through retention of the scFv protein in the endoplasmic reticulum. Recombinant antibodies accumulated up to 2% of total soluble tuber protein. After 1.5 years of tuber storage at 4 °C still half of the amount of scFv present in freshly harvested tubers was detectable. Its specific activity did not decrease during tuber storage. Recombinant protein could be efficiently purified from crude extracts by affinity chromatography.

antibody endoplasmic reticulum phytofarming potato production scFv 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Olga Artsaenko
    • 1
  • Barbara Kettig
    • 2
  • Ulrike Fiedler
    • 1
  • Udo Conrad
    • 1
  • Klaus Düring
    • 2
  1. 1.Institut für Pflanzengenetik und KulturpflanzenforschungGasterslebenGermany
  2. 2.Federal Centre for Breeding Research on Cultivated PlantsInstitute for Breeding Methods in VegetablesQuedlinburgGermany

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