Molecular Breeding

, Volume 6, Issue 1, pp 47–53 | Cite as

Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants

  • Adilson Leite
  • Edson L. Kemper
  • Márcio J. da Silva
  • Augusto D. Luchessi
  • Rodrigo M.P. Siloto
  • Eric D. Bonaccorsi
  • Hamza F. El-Dorry
  • Paulo Arruda
Article

Abstract

Human growth hormone was expressed in transgenic tobacco seeds using the monocot tissue-specific promoter from sorghum γ-kafirin seed storage protein gene. During tobacco seed ripening, the expressed hormone was directed to the endoplasmic reticulum by a signal peptide from a Coix prolamin and was secreted into the apoplastic space, where it accounted for 0.16% of total soluble seed protein. The expressed hormone has the same amino acid sequence and receptor-binding properties as the native mature hormone.

seed-specific expression human growth hormone plant farming heterologous protein transgenic plants 

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Adilson Leite
    • 1
  • Edson L. Kemper
    • 1
  • Márcio J. da Silva
    • 1
  • Augusto D. Luchessi
    • 1
  • Rodrigo M.P. Siloto
    • 1
  • Eric D. Bonaccorsi
    • 1
  • Hamza F. El-Dorry
    • 1
  • Paulo Arruda
    • 1
  1. 1.Departamento de Bioquímica, Instituto de QuímicaUniversidade de São PauloSão PauloBrasil

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