Molecular Breeding

, Volume 6, Issue 1, pp 25–36 | Cite as

Genetic mapping from field tests of qualitative and quantitative resistance to Phytophthora infestans in a population derived from Solanum tuberosum and Solanum berthaultii

  • Elmer E. Ewing
  • Ivan Šimko
  • Christine D. Smart
  • Merideth W. Bonierbale
  • Eduardo S.G. Mizubuti
  • Gregory D. May
  • William E. Fry


Under controlled field conditions, a Solanum backcross population segregated for resistance to Phytophthora infestans. The population (`BCT') had been derived previously by crossing the Solanum tuberosum dihaploid USW2230 × Solanum berthaultii PI473331 to obtain the hybrid M200-30, and then backcrossing the hybrid to the S. tuberosum dihaploid HH1-9. Resistance was assessed from analyses of epidemics in small plots of each individual genotype, and data were recorded as area under the disease progress curve (AUDPC). The parents of the original cross (USW2230 and a selection from PI473331) were not included in the test, but the hybrid was incompatible and HH1-9 was compatible with the tester strain of P. infestans (US-8 lineage). Somewhat more than half of the progeny also were incompatible with the tester strain, indicating the presence of an R gene. This gene segregated from the S. berthaultii parent and mapped 4.8 cm from the RFLP marker TG63 on chromosome 10. We deduce that the R gene is not R-1, R-2, R-3, R-6, or R-7 and is probably not R-4, R-5, or R-10. Among the remaining, compatible progeny, there was a wide range of quantitative resistance. All were more resistant than the susceptible cultivar Superior, and most individuals were much more resistant than the moderately resistant cultivar Kennebec. AUDPC values among the sub-population of compatible genotypes ranged from about 400 to 1500 units the first year and from 400 to 1760 units the second year. At least five quantitative trait loci (QTLs) were detected in this sub-population in both 1997 and 1998, including one detected through segregation of alleles from both the hybrid parent and the recurrent S. tuberosum parent. A model of main and epistatic effects explained 56% and 66% of the variation observed for quantitative resistance to late blight in 1997 and 1998, respectively. Several of the QTLs for late blight resistance were located in regions of the genome to which QTLs for late maturity have previously been mapped.

late blight Phytophthora infestans potato resistance,Solanum berthaultii Solanum tuberosum 


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  1. 1.
    Anonymous: The measurement of potato blight. Trans Br Mycol Soc 31: 140-141 (1947).Google Scholar
  2. 2.
    Bonierbale MW, Plaisted RL, Pineda O, Tanksley SD: QTL analysis of trichome-mediated insect resistance in potato. Theor Appl Genet 87: 973–987 (1994).Google Scholar
  3. 3.
    Chetelat RT, DeVerna JW: Expression of unilateral incompatibility in pollen of Lycopersicon pennellii is determined by major loci on chromosomes 1, 6 and 10. Theor Appl Genet 82: 704–712 (1991).Google Scholar
  4. 4.
    Colon L: Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species. PhD thesis, Wageningen Agricultural University, 159 pp. Ponsen & Looijen, Wageningen, Netherlands (1994).Google Scholar
  5. 5.
    Colon LT, Budding DJ: Resistance to late blight (Phytophthora infestans) in ten wild Solanum species. Euphytica S: 77-86 (1988).Google Scholar
  6. 6.
    Colon LT, Budding DJ, Keizer LCM, Pieters MMJ: Components of resistance to late blight (Phytophthora infestans) in eight South American Solanum species. Eur J Plant Pathol 101: 441–456 (1995).Google Scholar
  7. 7.
    Colon LT, Jansen RC, Budding DJ: Partial resistance to late blight (Phytophthora infestans) in hybrid progenies of four South American species crossed with diploid S. tuberosum. Theor Appl Genet 90: 691–698 (1995).Google Scholar
  8. 8.
    Colon LT, Turkensteen LJ, Prummel W, Budding DJ, Hoogendoorn J: Durable resistance to late blight (Phytophthora infestans) in old potato cultivars. Eur J Plant Pathol 101: 387–397 (1995).Google Scholar
  9. 9.
    de Jong W, Forsyth A, Leister D, Gebhardt C, Baulcombe DC: A potato hypersensitive resistance gene against potato virus X maps to a resistance gene cluster on chromosome 5. Theor Appl Genet 95: 246–252 (1997).Google Scholar
  10. 10.
    El-Kharbotly A, Leonards-Schippers C, Huigen D, Jacobsen E, Pereira A, Stiekema W, Salamini F, Gebhardt C: Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242: 749–754 (1994).Google Scholar
  11. 11.
    El-Kharbotly A, Palomino-Sanchez C, Salamini F, Jacobsen E, Gebhardt C: R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI. Theor Appl Genet 92: 880–884 (1996).Google Scholar
  12. 12.
    Ewing EE, Struik, PC: Tuber formation in potato: induction, initiation, and growth. Hort Rev 14: 89–198 (1992).Google Scholar
  13. 13.
    Foolad MR: Unilateral incompatibility as a major cause of skewed segregation in the cross between Lycopersicon esculentum and L. pennellii. Plant Cell Rep 15: 627–633 (1996).Google Scholar
  14. 14.
    Fry WE: Quantification of general resistance of potato cultivars and fungicide effects for integrated control of late blight. Phytopathology 68: 1650–1655 (1978).Google Scholar
  15. 15.
    Fry WE, Apple AE: Disease management implications of age-related changes in susceptibility of potato foliage to Phytophthora infestans. Am Potato J 63: 47–56 (1986).Google Scholar
  16. 16.
    Fry WE, Goodwin SB: Re-emergence of potato and tomato late blight in the United States. Plant Dis 81: 1349–1357 (1997).Google Scholar
  17. 17.
    Fry WE, Goodwin SB: Resurgence of the Irish potato famine fungus. Bioscience 47: 363–371 (1997).Google Scholar
  18. 18.
    Fry WE, Goodwin SB, Dyer AT, Matuszak JM, Drenth A, Tooley PW, Sujkowski LS, Koh YJ, Cohen BA, Spielman LJ, Deahl KL, Inglis DA, Sandlan KP: Historical and recent migrations of Phytophthora infestans: chronology, pathways, and implications. Plant Dis 77: 653–661 (1993).Google Scholar
  19. 19.
    Goodwin SB, Sujkowski LS, Dyer AT, Fry BA, Fry WE: Direct detection of gene flow and probable sexual reproduction of Phytophthora infestans in northern North America. Phytopathology 85: 473–479 (1995).Google Scholar
  20. 20.
    Goodwin SB, Sujkowski LS, Fry WE: Rapid evolution of pathogenicity within clonal lineages of the potato late blight disease fungus. Phytopathology 85: 669–676 (1995).Google Scholar
  21. 21.
    Hodgson WA: Laboratory testing of the potato for partial resistance to Phytophthora infestans. Am Potato J 38: 259–264 (1961).Google Scholar
  22. 22.
    Hunt GJ, Guzmán-Novoa E, Fondrk MK, Page RE Jr: Quantitative trait loci for honey bee stinging behavior and body size. Genetics 148: 1203–1213 (1998).Google Scholar
  23. 23.
    James WC: An illustrated series of assessment keys for plant diseases, their preparation and usage. Can Plant Dis Surv 51: 39–65 (1971).Google Scholar
  24. 24.
    Kato M, Mizubuti ESG, Goodwin SB, Fry WE: Sensitivity to protectant fungicides and pathogenic fitness of clonal lineages of Phytophthora infestans in the United States. Phytopathology 87: 973–978 (1997).Google Scholar
  25. 25.
    Kreike CM, De Koning JRA, Vinke JH, Van Ooijen JW, Gebhardt C, Stiekema WJ: Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype R01. Theor Appl Genet 87: 464–470 (1993).Google Scholar
  26. 26.
    Kreike CM, de Koning JRA, Vinke JH, Van Ooijen JW, Stiekema WJ: Quantitatively inherited resistance to Globodera pallida is dominated by one major locus in Solanum spegazzinii. Theor Appl Genet 88: 764–769 (1994).Google Scholar
  27. 27.
    Kreike CM, Kok-Westeneng AA, Vinke JH, Stiekema WJ: Mapping of QTLs involved in nematode resistance, tuber yield and root development in Solanum spp. Theor Appl Genet 92: 463–470 (1996).Google Scholar
  28. 28.
    Leister D, Ballvora A, Salamini F, Gebhardt C: A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants. Nature Genet 14: 421–429 (1996).Google Scholar
  29. 29.
    Leonard-Schippers C, Gieffers W, Salamini F, Gebhardt C: The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato chromosome V. Mol Gen Genet 223: 278–283 (1992).Google Scholar
  30. 30.
    Leonard-Schippers C, Gieffers W, Schafer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C: Quantitative resistance to Phytophthora infestans in potato: a case study for QTL mapping in an allogamous plant species. Genetics 137: 67–77 (1994).Google Scholar
  31. 31.
    Li X, Van Eck HJ, Rouppe van der Voort JNAM, Huigen DJ, Stam P, Jacobsen E: Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet 96: 1121–1128 (1998).Google Scholar
  32. 32.
    Lowings PH, Acha IG: Some factors affecting growth of Phytophthora infestans (Mont.) de Bary. 1. P. infestans on living potato leaves. Trans Br Mycol Soc 42: 491–501 (1959).Google Scholar
  33. 33.
    Malcolmson JF, Black W: New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de Bary. Euphytica 15: 199–203 (1966).Google Scholar
  34. 34.
    Manly KF: A Macintosh program for storage and analysis of experimental genetic mapping data. Mamm Genome 4: 303–313 (1993).Google Scholar
  35. 35.
    Meyer RC, Milbourne D, Hackett CA, Bradshaw JE, McNichol JW, Waugh R: Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans). Mol Gen Genet 259: 150–160 (1998).Google Scholar
  36. 36.
    Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia CR, Sasaki T: Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breed 3: 87–103 (1997).Google Scholar
  37. 37.
    Moreau P, Thoquet P, Olivier J, Laterrot H, Grimsely N: Genetic mapping of Ph-2, a single locus controlling partial resistance to Phytophthora infestans in tomato. Mol Plant-Microbe Interactions 11: 259–269 (1998).Google Scholar
  38. 38.
    Nelson J: QGENE: Software for marker-based genomic analysis and breeding. Mol Breed 3: 239–245 (1997).Google Scholar
  39. 39.
    Rouppe van der Voort J, Lindeman W, Folkertsma R, Hutten R, Overmars H, van der Vossen E, Jacobsen E, Bakker J: A QTL for broad-spectrum resistance to cyst nematode species (Globodera spp.) maps to a resistance gene cluster in potato. Theor Appl Genet 96: 654–661 (1998).Google Scholar
  40. 40.
    Shaner G, Finney RE: The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67: 1051–1056 (1977).Google Scholar
  41. 41.
    Šimko I, McMurry S, Yang HM, Manschot A, Davies PJ, Ewing EE: Evidence from polygene mapping for a causal relationship between potato tuber dormancy and abscisic acid content. Plant Physiol 115: 1453–1459 (1997).Google Scholar
  42. 42.
    Šimko I, Vreugdenhil D, Jung CS, May GD: Similarity of QTLs detected for in vitro and greenhouse development of potato plants. Mol Breed 5: 417–428 (1999).Google Scholar
  43. 43.
    Simmonds NW, Wastie RL: Assessment of horizontal resistance to late blight of potatoes. Ann Appl Biol 111: 213–221 (1987).Google Scholar
  44. 44.
    Swiezynski KM: Resistance to Phytophthora infestans in potato cultivars and its relation to maturity. Genet Pol 31: 99–106 (1990).Google Scholar
  45. 45.
    Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND: High density molecular linkage maps of the tomato and potato genomes. Genetics 132: 1141–1160 (1992).Google Scholar
  46. 46.
    Toxopeus HJ: Some notes on the relationship between field resistance to Phytophthora infestans in leaves and tubers and ripening time in Solanum tuberosum subsp. tuberosum. Euphytica 7: 123–130 (1958).Google Scholar
  47. 47.
    Trognitz BR: Inheritance of resistance in potato to lesion expansion and sporulation by Phytophthora infestans. Plant Pathol 47: 712–722 (1998).Google Scholar
  48. 48.
    Turkensteen LJ: Durable resistance of potatoes against Phytophthora infestans. In: Jacobs Th, Parlevliet JE (eds) Durability of Disease Resistance, pp. 115–124. Kluwer Academic Publishers, Dordrecht, Netherlands (1993).Google Scholar
  49. 49.
    Umaerus V, Umaerus M: Inheritance of resistance to late blight. In: Mackay GR (ed) Inheritance of resistance to late blight, pp. 365–401. CAB International, Wallingford, UK (1994).Google Scholar
  50. 50.
    Umaerus V, Umaerus M, Erjefalt L, Nilsson BA: Control of Phytophthora by host resistance: Problems and progress. In: Erwin DC, Bartnicki-Garcia S, Tsao PH (eds) Phytophthora: Its Biology, Taxonomy, Ecology and Pathology, pp. 315–326. American Phytopathological Society, St. Paul, MN (1983).Google Scholar
  51. 51.
    van den Berg JH, Ewing EE, Plaisted RL, McMurry S, Bonierbale MW: QTL analysis of potato tuberization. Theor Appl Genet 93: 307–316 (1996).Google Scholar
  52. 52.
    van den Berg JH, Ewing EE, Plaisted RL, McMurry S, Bonierbale MW: QTL analysis of potato tuber dormancy. Theor Appl Genet 93: 317–324 (1996).Google Scholar
  53. 53.
    van Eck HJ, Jacobsen E: Application of molecular markers in the genetic analysis of quantitative traits. In: Struik PC, Hoogendoorn J, Kouwenhoven JK, Mastenbroek LJ, Turkensteen LJ, Veerman A, Vos J (eds) Abstracts of conference papers, posters and demonstrations of the 13th Triennial conference of the EAPR, pp. 130–131. European Association for Potato Research, Wageningen (1996).Google Scholar
  54. 54.
    Van Soest LJM, Schober B, Tazelaar MF: Resistance to Phytophthora infestans in tuber-bearing species of Solanum and its geographical distribution. Potato Res 27: 393–411 (1984).Google Scholar
  55. 55.
    Wastie RL: Breeding for resistance. In: Ingram DS, Williams PH (eds) Breeding for Resistance, pp. 193–224. Academic Press, San Diego, CA (1991).Google Scholar
  56. 56.
    Yencho GC, Kowalski SP, Kobayashi RS, Sinden SL, Bonierbale MW, Deahl KL: QTL mapping of foliar glycoalkaloid aglycones in Solanum tuberosum × S. berthaultii potato progenies: quantitative variation and plant secondary metabolism. Theor Appl Genet 97: 563–574 (1998).Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Elmer E. Ewing
    • 1
  • Ivan Šimko
    • 1
  • Christine D. Smart
    • 2
  • Merideth W. Bonierbale
    • 3
  • Eduardo S.G. Mizubuti
    • 2
  • Gregory D. May
    • 4
  • William E. Fry
    • 2
  1. 1.Department of Fruit and Vegetable ScienceCornell UniversityIthacaUSA
  2. 2.Department of Plant PathologyCornell UniversityIthacaUSA
  3. 3.Centro Internacional de la Papa (CIP)Peru
  4. 4.Boyce Thompson Institute for Plant ResearchIthacaUSA

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