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Molecular Breeding

, Volume 5, Issue 2, pp 95–102 | Cite as

Resistance to African cassava mosaic virus conferred by a mutant of the putative NTP-binding domain of the Rep gene (AC1) in Nicotiana benthamiana

  • Abdourahamane Sangaré
  • Dalun Deng
  • Claude M. Fauquet
  • Roger N. Beachy
Article

Abstract

We constructed a mutation in DNA A of African cassava mosaic virus (ACMV) to alter the putative NTP-binding site in the replication- associated protein gene (AC1). When transgenic Nicotiana benthamiana plants expressing the mutated AC1 gene were infected with ACMV, the plants exhibited tolerance to infection consisting in a delay in symptom appearance and/or the presence of mild symptoms. In addition, the resistant plants accumulated less viral DNA than non-transgenic plants. As judged by northern blot analysis and symptom development of segregating progeny from different lines, a high level of expression of the mutated AC1 gene is essential for the development of resistance. Issues related to the use of different versions of AC1 for the control of ACMV are discussed.

AC1 mutant ACMV pathogen-derived resistance NTP-binding site 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Abdourahamane Sangaré
    • 1
  • Dalun Deng
    • 2
  • Claude M. Fauquet
    • 2
  • Roger N. Beachy
    • 2
  1. 1.Laboratoire de Génétique de l'UFR des BiosciencesUniversité de Cocody, 22Ivory Coast
  2. 2.Division of Plant BiologyILTAB/TSRILa JollaUSA

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