Advertisement

Journal of Assisted Reproduction and Genetics

, Volume 17, Issue 1, pp 39–43 | Cite as

In Vitro Maturation and Fertilization of Immature Oocytes: A Comparative Study of Fertilization Techniques

  • Jiann-Loung Hwang
  • Yu-Hung Lin
  • Yieh-Loong Tsai
Article

Abstract

Purpose: Our purpose was to investigate the factorsinfluencing maturation and fertilization of immature oocytes.

Methods: Immature oocytes were obtained from womenundergoing cesarean section. They were cultured in thematuration medium either with or without cumulus cells. Aftermaturation to metaphase II, they were randomly fertilizedby in vitro fertilization (IVF) or intracytoplasmic sperminjection (ICSI).

Results: After incubation for 48 hr, 441 oocytes (42.8%)reached metaphase II. Among them, 56.6% ofcumulus-enclosed oocytes, but only 29.2% of denuded oocytes,reached metaphase II. Of the 289 cumulus-enclosed oocytes,the fertilization rates by IVF and ICSI were 56.3 and 84.1%,respectively (P < 0.01). Of the 152 denuded oocytes, thefertilization rates by IVF and ICSI were 39.5 and 84.5%,respectively (P < 0.01). The cleavage rates, however,were similar.

Conclusions: Cumulus cells are beneficial in the maturationof human oocytes in vitro and that ICSI increases thefertilization rate for the in vitro matured oocytes. Thedevelopmental potential of the fertilized oocytes, however, is similarirrespective of the fertilization method or the presence orabsence of cumulus cells.

immature oocytes in vitro maturation cumulus cells fertilization intracytoplasmic sperm injection 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

REFERENCES

  1. 1.
    Edwards RG: Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature 1965; 208:349-351Google Scholar
  2. 2.
    Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Woon TK: Pregnancy after in vitro fertilization of human follicular oocytes collected fertilizafrom nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991;55:109-113Google Scholar
  3. 3.
    Trounson A, Bongso A, Szell A, Barnes FL: Maturation of human and bovine primary oocytes in vitro for fertilization and embryoproduction. Singapore J Obstet Gynecol 1996;27:78-84Google Scholar
  4. 4.
    Eppig J: A comparison between oocyte growth in coculture with granulosa cells and oocytes with granulosa cell-oocyte junctional contact maintained in vitro. J Exp Zool 1979; 209:345-353Google Scholar
  5. 5.
    Vanderhyden BC, Armstrong DT: Role of cumulus cells and serum on the in vitro maturation, fertilization, and subsequent development of rat oocytes. Biol Reprod 1989;40:720-728Google Scholar
  6. 6.
    Chian RC, Niwa K, Sirard MA: Effects of cumulus cells on male pronuclear formation and subsequent early development of bovine oocytes in vitro. Theriogenology 1994;41:1499-1508Google Scholar
  7. 7.
    Barnes FL, Kausche A, Tiglias J, Wood C, Wilton L, Trounson A: Production of embryos from in vitro-matured primary human oocytes. Fertil Steril 1996;65:1151-1156Google Scholar
  8. 8.
    Palermo G, Joris H, Derde MP, Camus M, Devroey P, Van Steirteghem A: Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertil Steril 1993;59:199-207Google Scholar
  9. 9.
    Trounson A, Wook C, Kausche A: In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62:353-362Google Scholar
  10. 10.
    Staigmiller RM, Moor RM: Effect of follicle cells on the maturation and developmental competence of ovine oocytes matured outside the follicle. Gamete Res 1984;9:221-229Google Scholar
  11. 11.
    Kennedy JF, Donahue RP: Human oocytes: Maturation in chemically defined media. Science 1969;164:1292-1293Google Scholar
  12. 12.
    Schroeder AC, Eppig JJ: The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev Biol 1984;102:493-397Google Scholar
  13. 13.
    Crosby IM, Osborn JC, Moor RM: Follicle cell regulation of protein synthesis and developmental competence in sheep oocytes. J Reprod Fertil 1981;62:575-582Google Scholar
  14. 14.
    Cha KY, Chian RC: Maturation in vitro of immature human oocytes for clinical use. Hum Reprod Update 1998;4: 103-120Google Scholar
  15. 15.
    Choi TS, Mori M, Kohmoto K, Shoda Y: Beneficial effect of serum on the fertilizability of mouse oocytes matured in vitro. J Reprod Fertil 1987;79:565-568Google Scholar
  16. 16.
    Leibfried-Rutledge ML, Critser ES, Eyestone WH, Northey DL, First NL: Development potential of bovine oocytes matured in vitro or in vivo. Biol Reprod 1987;36:376-383Google Scholar
  17. 17.
    Moor RM, Trounson AO: Hormonal and follicular factors affecting maturation of sheep oocytes in vitro and their subsequent developmental capacity. J Reprod Fertil 1977; 49:101-109Google Scholar
  18. 18.
    Russel JB, Knezevich KM, Fabian KF, Dickson JA: Unstimulated immature oocytes retrieval: Early versus midfollicular endometrial priming. Fertil Steril 1997;67:616-620Google Scholar
  19. 19.
    Green DPL: Three-dimensional structure of the zona pellucida. Rev Reprod 1997;2:147-156Google Scholar

Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Jiann-Loung Hwang
    • 1
  • Yu-Hung Lin
    • 1
  • Yieh-Loong Tsai
    • 1
  1. 1.Department of Obstetrics and GynecologyShin-Kong Wu Ho-Su Memorial HospitalTaipeiTaiwan

Personalised recommendations