Journal of Fluorescence

, Volume 10, Issue 3, pp 229–229 | Cite as

Energy Transfer from the Aminophthalate Dianion to Fluorescein

  • Mariana Voicescu
  • Marilena Vasilescu
  • Aurelia Meghea
Article

Abstract

Energy transfer from the excited aminophthalate dianion species to fluorescein at pH 8.32 (Tris-HCl buffer) was studied. The excited aminophthalate dianion species was obtained either by excitation with UV radiation (330 nm), with fluorescence emission, or by the well-known chemical reaction luminol-hydrogen peroxide in an alkaline medium, with chemiluminescent emission, both with λmax at 425 nm. The influence of Co2+ and Mg2+ on fluorescence and chemiluminescence (CL) was studied. It was found that at low concentrations (10−7−10−9M), these ions do not modify the fluorescein fluorescence, however, the CL is strong affected. The effect of the concentration of these elements, which exert an influence on CL even at a high dilution (nanomolar concentration), was determined. In the case of Co2+ the prooxidant character is stronger than in the case of Mg2+, and therefore the CL enhancer effect is higher. Compared to the system without catalyst, their presence ensures stronger, prolonged, and stable light emission. The emission spectra, in the presence of fluorescein, show two bands with maxima at 425 and 520 nm, the second one being specific to fluorescein emission. The intensity of aminophthalate dianion luminescence is lower and the duration shorter in the presence of fluorescein. The influence of Co2+ and Mg2+ catalyst and fluorescein concentration on the energy transfer process was studied. The efficiency of the energy transfer process for these two situations (fluorescence and CL) was compared. An attempt was made to replace hydrogen peroxide with superoxide anion (solubilized by means of crown ether) and its effect upon the energy transfer process was observed.

Energy transfer aminophthalate dianion fluorescein chemiluminescence 

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Copyright information

© Plenum Publishing Corporation 2000

Authors and Affiliations

  • Mariana Voicescu
    • 1
  • Marilena Vasilescu
    • 1
  • Aurelia Meghea
    • 2
  1. 1.Institute of Physical ChemistryBucharestRomania
  2. 2.Department of Apllied Physical Chemistry and ElectrochemistryPolytehnic UniversityBucharestRomania

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