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Chromosome Research

, Volume 6, Issue 7, pp 567–574 | Cite as

Comparative Analysis of Crossover Exchanges and Chiasmata in Allium Cepa × Fistulosum After Genomic In Situ Hybridization (GISH)

  • M. Stevenson
  • S. J. Armstrong
  • B. V. Ford-Lloyd
  • G. H. Jones
Article

Abstract

Genomic in situ hybridization (GISH) successfully differentiated homoeologous genomes in the interspecific hybrid Allium cepa × fistulosum, thus allowing the detection of reciprocal crossover events as label exchanges in separating anaphase I chromosomes. Three of the eight chromosome pairs were positively identified by fluorescence in situ hybridization (FISH) to rDNA sequences. There was a general similarity of the GISH-based label exchange frequencies and metaphase I chiasma frequencies, but with a 20% deficit of chiasmata. Reasons for this apparent deficit are discussed. The locations of chiasmata and label exchanges are in broad agreement.

Allium chiasmata crossing over genomic in situ hybridization recombination 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • M. Stevenson
    • 1
  • S. J. Armstrong
    • 1
  • B. V. Ford-Lloyd
    • 1
  • G. H. Jones
    • 1
  1. 1.School of Biological SciencesThe University of BirminghamEdgbaston, BirminghamUK

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