Chromosome Research

, Volume 7, Issue 8, pp 655–662 | Cite as

Classical and Molecular Cytogenetics of the Pufferfish Tetraodon Nigroviridis

  • Frank Grützner
  • Götz Lütjens
  • Carlos Rovira
  • David W. Barnes
  • Hans-Hilger Ropers
  • Thomas Haaf

Abstract

Because of its highly compact genome, the pufferfish has become an important animal model in genome research. Although the small chromosome size renders chromosome analysis difficult, we have established both classical and molecular cytogenetics in the freshwater pufferfish Tetraodon nigroviridis (TNI). The karyotype of T. nigroviridis consists of 2n = 42 biarmed chromosomes, in contrast to the known 2n = 44 chromosomes of the Japanese pufferfish Fugu rubripes (FRU). RBA banding can identify homologous chromosomes in both species. TNI 1 corresponds to two smaller FRU chromosomes, explaining the difference in chromosome number. TNI 2 is homologous to FRU 1. Fluorescence in-situ hybridization (FISH) allows one to map single-copy sequences, i.e. the Huntingtin gene, on chromosomes of the species of origin and also on chromosomes of the heterologous pufferfish species. Hybridization of total genomic DNA shows large blocks of (species-specific) repetitive sequences in the pericentromeric region of all TNI and FRU chromosomes. Hybridization with cloned human rDNA and classical silver staining reveal two large and actively transcribed rRNA gene clusters. Similar to the situation in mammals, the highly compact pufferfish genome is endowed with considerable amounts of localized repeat DNAs.

FISH Fugu rubripes heterochromatin Huntingtin NOR pufferfish replication banding Tetraodon nigroviridis 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Frank Grützner
    • 1
  • Götz Lütjens
    • 1
  • Carlos Rovira
    • 2
  • David W. Barnes
    • 3
  • Hans-Hilger Ropers
    • 1
  • Thomas Haaf
    • 1
  1. 1.Max-Planck-Institute of Molecular GeneticsBerlinGermany
  2. 2.Department of Biosciences at NovumKarolinska InstituteHuddingeSweden
  3. 3.ATCCManassasUSA

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