Chromosome Painting in Marsupials: Genome Conservation in the Kangaroo Family
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In order to deduce the ancestral genome arrangement in the karyotypically diverse marsupial family Macropodidae, and to assess chromosome change in this family, chromosome-specific paints from the tammar wallaby (2n = 16) were hybridized to metaphase spreads from the two species proposed to represent the 2n = 22 ancestral karyotype, as well as species with derived 2n = 20 and 2n = 14 karyotypes. Identical patterns were observed in the two 2n = 22 species, from which the rearrangements to form the three derived karyotypes may be easily deduced to be 1, 3 and 4 different fusions, respectively. The identical Thylogale and Dorcopsis genomes may both be used to represent the pleisiomorphic macropodid chromosome complement. Variation in the X chromosome was also investigated by hybridizing an X-Y shared tammar wallaby 12-kb repeat element to chromosomes from the other four macropodid species, finding that it hybridized only to the most closely related species, and therefore is of recent origin.
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- Flannery TF (1989) Phylogeny of the Macropoidea; a study in convergence. In: Grigg G, Jarman P, Hume I, eds. Kangaroos, Wallabies and Rat-kangaroos, Vol. 1.Google Scholar
- Hayman DL (1977) Chromosome number — constancy and variation. In: Stonehouse B, Gilmore D eds. The Biology of Marsupials. Baltimore: University Park Press, pp 27-48.Google Scholar
- Hayman DL (1990) Marsupial cytogenetics. In: Graves JAM, Hope RM, Cooper DW, eds. Mammals from Pouches and Eggs: Genetics, Breeding Evolution of Marsupials and Monotremes, Melbourne: CSIRO, pp 189-208.Google Scholar
- Hayman DL, Martin PG (1974) Mammalia I: Monotremata and Marsupialia. In: John B, ed. Animal Cytogenetics, Vol 4. 1-109. Berlin, Stuttgart: Gebruder Borntraeger, pp 1–109.Google Scholar
- Ohno S (1967) Sex Chromosomes and Sex-linked Genes. Berlin: Springer-Verlag.Google Scholar
- O'Neill RJW, Eldridge MDB, Toder R, Ferguson-Smith MA, O'Brien PCM, Graves JAM (1999) Chromosome evolution in kangaroos (Marsupialia: Macropodidae). Cross species chromosome painting between the tammar wallaby and rock wallaby spp. with the 2n = 22 ancestral macropodid karyotype. Genome (in press).Google Scholar
- Rofe RH (1978) G-banded chromosomes and the evolution of the Macropodidae. Aust Mammal 2: 53-63.Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning, a Laboratory Manual. Cold Spring Harbour Laboratory Press.Google Scholar
- Szalay FS (1994) Evolutionary History of the Marsupials and an Analysis of Osteological Characters. New York: Cambridge University Press.Google Scholar
- Telenius H, Pelmear AH, Tunnacliffe A et al. (1992) Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosome Cancer 4: 257-263.Google Scholar
- Westerman M, Woolley PA (1993) Chromosomes and the evolution of Dasyurid marsupials: An overview. Sci New Guinea 19: 123-130.Google Scholar
- Young GJ, Graves JMG, Barbieri I, Woolley PA, Cooper DW, Westerman M (1982) The chromosomes of dasyurids (Marsupialia). In: Archer M, ed. Carnivorous Marsupials, Vol 2. Chipping Norton, NSW: Royal Zool. Soc. NSW. pp 783-795.Google Scholar