Chromosome Research

, Volume 8, Issue 7, pp 603–613 | Cite as

A biodiversity approach in the neotropical Erythrinidae fish, Hoplias malabaricus. Karyotypic survey, geographic distribution of cytotypes and cytotaxonomic considerations

  • Luiz A. C. Bertollo
  • Guassenir Gonçalves Born
  • Jorge A. Dergam
  • Alberto Sergio Fenocchio
  • Orlando Moreira-Filho
Article

Abstract

Hoplias malabaricus, a widely distributed neotropical freshwater fish, shows a conspicuous karyotypic diversification. An overview of this diversity is presented here comprising several Brazilian populations, and some others from Argentina, Uruguay and Surinam. Seven general cytotypes are clearly identified on the basis of their diploid number (2n=39 to 2n=42), chromosomal morphology and sex chromosome systems, which can be clustered into two major karyotypic groups. This clustering suggests that karyotype structure would be more informative than the diploid number regarding cytotype relationships in this fish group. While some cytotypes show a wide geographical distribution, some others appear to be endemic to specific hydrographic basins. Sympatric cytotypes can occur without detection of hybrid forms; this situation points to a lack of gene flow, a fact that is also reinforced by studies with genomic markers. The karyotypic data support the view that the nominal taxon H. malabaricus corresponds to a species complex comprising distinct evolutionary units, each with well-established chromosomal differences.

cytotaxonomy geographic distribution Hoplias malabaricus fish karyotypic diversity sympatric cytotypes 

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References

  1. Bertollo LAC, Mestriner CA (1998) The X1X2Y sex chromosome system in the fish Hoplias malabaricus (Pisces, Erythrinidae). II. Meiotic analyses. Chromosome Res 6: 141–147.Google Scholar
  2. Bertollo LAC, Takahashi CS, Moreira-Filho O (1978) Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). Brazil J Genet 1: 103–120.Google Scholar
  3. Bertollo LAC, Mestriner CA (1998) The X1X2Y sex chromosome system in the fish Hoplias malabaricus (Pisces, Erythrinidae). II. Meiotic analyses. Chromosome Res 6: 141–147.Google Scholar
  4. Bertollo LAC, Takahashi CS, Moreira-Filho O (1978) Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). Brazil J Genet 1: 103–120.Google Scholar
  5. Bertollo LAC, Takahashi CS, Moreira-Filho O (1979) Karyotypic studies of two allopatric populations of the genus Hoplias (Pisces, Erythrinidae). Brazil J Genet 2: 17–37.Google Scholar
  6. Bertollo LAC, Takahashi CS, Moreira-Filho O (1983) Multiple sex chromosomes in the genus Hoplias (Pisces, Erythrinidae). Cytologia 48: 1–12.Google Scholar
  7. Bertollo LAC, Moreira-Filho O, Galetti Jr PM (1986) Cytogenetics and taxonomy: considerations based on chromosome studies of freshwater fish. J Fish Biol 28: 153–159.Google Scholar
  8. Bertollo LAC, Moreira-Filho O, Fontes MS (1997a) Karyotypic diversity and distribution in Hoplias malabaricus (Pisces, Erythrinidae): Cytotypes with 2n = 40 chromosomes. Brazil J Genet 20: 237–242.Google Scholar
  9. Bertollo LAC, Fontes MS, Fenocchio AS, Cano J (1997b) The X1X2Y sex chromosome system in the fish Hoplias malabaricus. I. G-, C-and chromosome replication banding. Chromosome Res 5: 493–499.Google Scholar
  10. Born GG, Bertollo LAC (2000) An XX/XY sex chromosome system in a fish species, Hoplias malabaricus, with a polymorphic NOR-bearing X chromosome. Chromosome Res 8: 111–118.Google Scholar
  11. Britski HA, Sato Y, Rosa ABS (1986) Manual de Identificação de Peixes da Região de Três Marias, 2nd edn. Brasília: Codevasf.Google Scholar
  12. Dergam JA (1996) Phylogeography and character congruence within the Hoplias malabaricus Bloch, 1794 (Erythrinidae, Characiformes, Ostariophysi) species complex. PhD thesis. Colorado State University, USA.Google Scholar
  13. Dergam JA, Bertollo LAC (1990) Karyotypic diversification in Hoplias malabaricus (Osteichthyes, Erythrinidae) of the São Francisco and Alto Paraná basins, Brazil. Brazil J Genet 13: 755–766.Google Scholar
  14. Dergam JÁ, Suzuki HI, Shibatta OA et al. (1998) Molecular biogeography of the Neotropical fish Hoplias malabaricus (Erythrinidae: Characiformes) in the Iguaçu, Tibagi, and Paraná rivers. Genet Mol Biol 21: 493–496.Google Scholar
  15. Fenocchio AS, Venere PC, Cesar ACG, Dias AL, Bertollo LAC (1991) Short term culture from solid tissues of fishes. Caryologia 44: 161–166.Google Scholar
  16. Ferreira RHR, Fonseca CG, Bertollo LAC, Foresti F (1989) Cytogenetics of fishes from Parque Florestal do Rio Doce (MG). I. Preliminary study of “Hopliasmalabaricus” (Pisces, Erythrinidae) from Lagoa Carioca and Lagoa dos Patos. Brazil J Genet 12: 219–226.Google Scholar
  17. Foresti F, Oliveira C, Almeida-Toledo LF (1993) A method for chromosome preparations from large fish specimens using in vitro short-term treatment with colchicine. Experientia 49: 810–813.Google Scholar
  18. Jorge LC (1995). Estudos citogenéticise comparativor de algumas espécies de peixes da região de Corrientes-Argentina com as do Alto Paraná. MSc dissertation. Universidade Federal de São Carlos, Brazil.Google Scholar
  19. Kligerman AD, Bloom SE (1977) Rapid chromosome preparations from solid tissues of fishes. J Fish Res Bd Can 34: 266–269.Google Scholar
  20. Lee MR, Elder FFB (1980) Yeast stimulation of bone marrow mitosis for cytogenetic investigations. Cytogenet Cell Genet 26: 36–40.Google Scholar
  21. Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52: 201–220.Google Scholar
  22. Lopes PA, Fenocchio AS (1994) Confirmation of two different cytotypes for the neotropical fish Hoplias malabaricus Gill 1903 (Characiformes). Cytobios 80: 217–221.Google Scholar
  23. Lopes PA, Alberdi AJ, Dergam JA, Fenocchio AS (1998) Cytotaxonomy of Hoplias malabaricus (Osteichthyes, Erythrinidae) in the Aguapey river (Province of Corrientes, Argentina). Copeia 1998: 485–487.Google Scholar
  24. Oyakawa OT (1990) Revisão sistemática das espécies do gênero Hoplias (grupo lacerdae)da Amazônia Brasileira e região leste do Brasil (Teleostei, Erythrinidae). MSc dissertation. Universidade de São Paulo, Brazil.Google Scholar
  25. Scavone MDP, Bertollo LAC, Cavallini MM (1994) Sympatric occurrence of two karyotypic forms of Hoplias malabaricus (Pisces, Erythrinidae). Cytobios 80: 223–227.Google Scholar
  26. Sites JW Jr, Moritz C (1987) Chromosomal evolution and speciation revisited. Syst Zool 36: 153–174.Google Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Luiz A. C. Bertollo
    • 1
  • Guassenir Gonçalves Born
    • 2
  • Jorge A. Dergam
    • 3
  • Alberto Sergio Fenocchio
    • 4
  • Orlando Moreira-Filho
    • 1
  1. 1.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão Carlos, SPBrazil
  2. 2.Departamento de Ciências MorfobiológicasUniversidade do Rio GrandeRio Grande, RSBrazil
  3. 3.Departamento de Biologia AnimalUniversidade Federal de ViçosaViçosa, MGBrazil
  4. 4.Departamento de GenéticaUniversidad Nacional de MisionesPosadasArgentina

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