Chromosome Research

, Volume 7, Issue 5, pp 363–367 | Cite as

Chromosomal Localization of 5S rDNA Genes in Leporinus Fish (Anostomidae, Characiformes)

  • Cesar Martins
  • Pedro M. GalettiJrEmail author


The large 45S rDNA chromosome sites have often been analyzed in fish. In contrast, little is known about the 5S genes in this animal group. In the genus Leporinus, the NOR chromosomal location has been shown to be very diverse. In the present work, chromosome mapping of 5S rDNA in three anostomids, Leporinus elongatus, L. obtusidens and L. friderici, is investigated using fluorescence in-situ hybridization (FISH) with PCR-obtained 5S probes and primed in-situ labeling (PRINS). Major 5S rDNA chromosomal sites were found to be subterminally located in a small metacentric pair, while minor ones were detected near the centromeric region of a medium-sized submetacentric pair in all studied species. The 5S rDNA genes were not associated with the NORs or sex chromosomes. A highly conserved chromosomal location of these genes appears to characterize the karyotype evolution of this fish group.

FISH fish chromosome Leporinus PRINS 5S rDNA 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bertollo LAC, Takahashi CS, Moreira Filho O (1978) Cytotaxonomic consideration on Hoplias Iacerdae (Pisces, Erythrinidae). Braz J Genet 1(2): 103-120.Google Scholar
  2. Castro J, Viñas A, Sánchez L, Martínez P (1996) Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta) with Ag-and CMA3-staining, and fluorescent in-situ hybridization. Cytogenet Cell Genet 75: 234-239.Google Scholar
  3. Christensen K, Lomholt B, Hallenberg C, Nielsen KU (1998) Mink 5S rRNA genes map to 2q in three loci suggesting conservation of synteny with human 1q. Hereditas 128: 17-20.Google Scholar
  4. Coullin P, Andréo B, Charlieu JP, Candelier J, Pellestor F (1997) Primed in situ (PRINS) labelling with Alu and satellite primers for rapid characterization of human chromosomes in hybrid cell lines. Chromosome Res 5: 307-312.Google Scholar
  5. Danna KJ, Workman R, Coryell V, Keim P (1996) 5S rRNA genes in tribe Phaseoleae: array size, number, and dynamics. Genome 39: 445-455.Google Scholar
  6. Dover GA (1986) Linkage disequilibrium and molecular drive in the rDNA gene family. Genetics 122: 249-252.Google Scholar
  7. Drouin G, Moniz de Sá M (1995) The concerted evolution of 5S ribosomal genes linked to the repeat units of other multigene families. Mol Biol Evol 12(3): 481-493.Google Scholar
  8. Galetti Jr PM (1998) Chromosome diversity in neotropical fish: NOR studies. Ital J Zool 65(suppl.): 53-56.Google Scholar
  9. Galetti Jr PM, Foresti F, Bertollo LAC, Moreira-Filho O (1984) Characterization of eight species of Anostomidae (Cypriniformes) fish on the basis of the nucleolar organizing region. Caryologia 37(4): 401-406.Google Scholar
  10. Galetti Jr PM, Cesar ACG, Venere PC (1991) Heterochromatin and NORs variability in Leporinus fish (Anostomidae, Characiformes). Caryologia 44(3–4): 287-292.Google Scholar
  11. Galetti Jr PM, Mestriner CA, Monaco PJ, Rasch EM (1995) Postzygotic modifications and intra-and inter-individual nucleolar organizing region variations in fish: report of a case involving Leporinus friderici. Chromosome Res 3: 285-290.Google Scholar
  12. Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014-1015.Google Scholar
  13. Kock EJ, Kolvraa S, Petersen KB, Gregersen N, Bolund L (1989) Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma 98: 259-265.Google Scholar
  14. Koehler MR, Dehm D, Guttenbach M et al. (1997) Cytogenetics of the genus Leporinus (Pisces, Anostomidae). 1. Karyotype analysis, heterochromatin distribution and sex chromosomes. Chromosome Res 5: 12-22.Google Scholar
  15. Komiya H, Hasegawa M, Takemura S (1986) Differentiation of oocyte-and somatic-type 5S rRNAs in animals. J Biochem 100: 369-374.Google Scholar
  16. Lomholt B, Frederiksen S, Nederby Nielsen J, Hallenberg C (1995) Additional assignment of the human 5S rRNA genes to chromosome region 1q31. Cytogenet Cell Genet 70: 76-79.Google Scholar
  17. Lomholt B, Frederiksen S, Jensen LR, Christensen K, Hallenberg C (1996) 5S rRNA genes in Macaca fascicularis map to chromosome 1p in three loci. Mamm Genome 7: 451-453.Google Scholar
  18. Lucchini S, Nardi I, Barsacchi G, Batistoni R, Andronico F (1993) Molecular cytogenetics of the ribosomal (18S + 28S and 5S) DNA loci in primitive and advanced urodele amphibians. Genome 36: 762-773.Google Scholar
  19. Mäkinem A, Zijlstra C, De Haan NA, Mellink CHM, Bosma AA (1997) Localization of 18S plus 28S and 5S ribosomal RNA genes in the dog by fluorescence in situ hybridization. Cytogenet Cell Genet 78(3–4): 231-235.Google Scholar
  20. Martínez JL, Morán P, García-Vázquez S, Pendás AM (1996) Chromosomal localization of the major and 5S rRNA genes in the European eel (Anguilla anguilla). Cytogenet Cell Genet 73: 149-152.Google Scholar
  21. Martins C, Galetti Jr PM (1997) Narrow chromosome diversity in fishes of the genus Schizodon (Characiformes, Anistomidae). Cytobios 92: 139-147.Google Scholar
  22. Martins C, Galetti Jr PM (1998) Karyotype similarity between two sympatric Schizodon fish species (Anostomidae, Characiformes) from the Paraguai River basin. Gen Mol Biol 21(3): 355-360.Google Scholar
  23. Molina WF, Galetti Jr PM (1998) Heterochromatin and sex chromosomes in the neotropical fish genus Leporinus (Characiformes, Anostomidae). Cytobios 94: 141-149.Google Scholar
  24. Morán P, Martínez JL, Garcia-Vázquez E, Pendás AM (1996) Sex linklage of 5S rDNA in rainbow trout (Oncorhynchus mykiss). Cytogenet Cell Genet 75: 145-150.Google Scholar
  25. Murakami M, Fujitani H (1998) Characterization of repetitive DNA sequences carrying 5S rDNA of the tripolid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi). Genes Genet Syst 73: 9-20.Google Scholar
  26. Pendás AM, Morán P, Freije JP, García-Vázquez E (1994) Chromosomal mapping and nucleotide sequence of two tandem repeats of Atlantic salmon 5S rDNA. Cytogenet Cell Genet 67: 31-36.Google Scholar
  27. Prado EA, Faivre-Rampant P, Schneider C, Darmency MA (1996) Detection of a variable number of ribosomal DNA loci by fluorescent in situ hybridization in Populus species. Genome 39: 1020-1026.Google Scholar
  28. Sajdak SL, Reed KM, Phillips RB (1998) Intraindividual and interspecies variation in the 5S rDNA of coregonid fish. J Mol Evol 46: 680-688.Google Scholar
  29. Schmid M, Vitelli L, Batistoni R (1987) Chromosome banding in Amphibia. IV. Constitutive heterochromatin, nucleolus organizers, 18S + 28S and 5S ribosomal RNA genes in Ascaphidae, Pipidae, Discoglossidae and Pelobatidae. Chromosoma 95: 271-284.Google Scholar
  30. Sola L, Galetti PM, Monaco PJ, Rasch EM (1997) Cytogenetics of bisexual/unisexual species of Poecilia. VI. Additional nucleolus organizer region chromosomal clones of Poecilia formosa (Amazon molly) from Texas, with a survey of chromosomal clones detected in Amazon molly. Heredity 78: 612-619.Google Scholar
  31. Suzuki H, Sakurai S, Matsuda Y (1996) Rat rDNA spacer sequences and chromosomal assignment of the genes to the extreme terminal region of chromosome 19. Cytogenet Cell Genet 72(1): 1-4.Google Scholar
  32. Vitelli L, Batistoni R, Andronico F, Nardi I, Barsacchi-Pilone G (1982) Chromosomal localization of 18S + 28S and 5S ribosomal RNA genes in evolutionary divergence anuran amphibians. Chromosoma 84: 475-491.Google Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  1. 1.Departamento de Genética e EvoluçãboUniversidade Federal de São CarlosSão Carlos, SPBrazil

Personalised recommendations