Aquaculture International

, Volume 8, Issue 4, pp 327–334

Developmental changes in structure and polypeptide profile of the androgenic gland of the freshwater prawn Macrobrachium rosenbergii

  • Piera S. Sun
  • Tina M. Weatherby
  • Marilyn F. Dunlap
  • Kristi L. Arakaki
  • Donis T. Zacarias
  • Spencer R. Malecha
Article

DOI: 10.1023/A:1009205027360

Cite this article as:
Sun, P.S., Weatherby, T.M., Dunlap, M.F. et al. Aquaculture International (2000) 8: 327. doi:10.1023/A:1009205027360

Abstract

Although the mechanism of sex-differentiation in crustaceans has yet to be defined, the androgenic gland (AG) is thought to be the exclusive organ that produces the androgenic hormone (AH) which induces male sexual development. This paper presents results of light and transmission electron microscopy and total protein analysis of androgenic glands from three male morphotypes (orange-claw, orange-blue-claw and blue-claw) of the freshwater prawn, Macrobrachium rosenbergii. Highest protein content (76 µg//AG) was found in the blue-claw morphotype as compared to the orange-blue-claw (45 µg/AG) and the orange-claw morphotype (19 µg/AG). Sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-page) analysis of the cell free extract of the AG from the three morphotypes revealed four polypeptides (∼16, ∼18, ∼23 and ∼26 Kd) which quantitatively increase from the sexually immature orange-claw to the sexually mature blue-claw morphotype. The 16 and 18 Kd polypeptides could be the AHs.

androgenic gland androgenic hormone freshwater prawn light and transmission electron microscopy (Macrobrachium rosenbergiimale morphotypes sex-differentiation sodium dodecyl sulfate polyacrylamide gel electrophoresis 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • Piera S. Sun
    • 1
  • Tina M. Weatherby
    • 1
  • Marilyn F. Dunlap
    • 1
  • Kristi L. Arakaki
    • 1
  • Donis T. Zacarias
    • 1
  • Spencer R. Malecha
    • 1
  1. 1.Pacific Biomedical Research CenterUniversity of Hawaii-ManoaHonoluluUSA

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