Chromosome Research

, Volume 7, Issue 3, pp 223–233

Localization and Characterization of Nucleotide Sequences From the Canine Y Chromosome

  • Michael Olivier
  • Matthew Breen
  • Matthew M. Binns
  • George Lust
Article

DOI: 10.1023/A:1009203500926

Cite this article as:
Olivier, M., Breen, M., Binns, M.M. et al. Chromosome Res (1999) 7: 223. doi:10.1023/A:1009203500926

Abstract

We previously reported the identification of a male-specific 658-bp DNA sequence in dogs. We used a specific primer pair designed for PCR amplification of this fragment with DNA samples from 238 dogs, 6 dingoes and 12 wolves. All 133 male samples amplified the 658-bp sequence, whereas all female samples did not. The sequence was not amplified from male DNA samples representing other wild canids (jackals, coyotes, foxes). A lambda phage was isolated from a canine male genomic library that contained an insert of approximately 15 kb of canine genomic DNA, including the male- specific 658-bp sequence. This lambda phage was used in fluorescence in-situ hybridization experiments. It hybridized to the canine Y chromosome together with a lambda clone containing a segment of the SRY gene and a cosmid clone containing a portion of the pseudoautosomal region. The male-specific 658-bp sequence was located at the end opposite to the pseudoautosomal region while the SRY gene sequence hybridized near the centromere.

Additionally, two (CA)-repeat sequences were identified in the lambda clone that contained the 658-bp sequence. Specific primer pairs were designed to amplify each of the repeats. Primer pair MS34 amplified three different alleles from 13 unrelated canine male DNA samples with a PIC value of 0.40. Primer pair MS41 amplified five alleles with a PIC value of 0.71. These microsatellites are the first reported polymorphic sequences in the dog located in the non-recombining portion of the Y chromosome.

dog FISH microsatellite Y chromosome 

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Michael Olivier
    • 1
    • 3
  • Matthew Breen
    • 2
  • Matthew M. Binns
    • 2
  • George Lust
    • 1
  1. 1.James A. Baker Institute for Animal HealthCollege of Veterinary Medicine, Cornell UniversityIthacaUSA and
  2. 2.Centre for Preventive Medicine, Animal Health TrustSuffolkUK
  3. 3.Department of Genetics M314Stanfor University Medical SchoolStanfordUSA

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