Russian Journal of Plant Physiology

, Volume 48, Issue 1, pp 1–11 | Cite as

Proteins of Axial Organs of Dormant and Germinating Horse Chestnut Seeds: 1. General Characterization

  • N. A. Gumilevskaya
  • M. I. Azarkovich
  • M. E. Komarova
  • N. V. Obroucheva


This is the first characterization of proteins from axial organs of recalcitrant horse chestnut seeds during deep dormancy, dormancy release, and germination. We demonstrated that, during the entire period of cold stratification, axial organs were enriched in easily soluble albumin-like proteins and almost devoid of globulins. About 80% of the total protein was found in the cytosol. Approximately one third of cytosolic proteins were heat-stable polypeptides, which were major components of total proteins. Heat-stable proteins comprised three groups of polypeptides with mol wts of 52–54, 24–25, and 6–12 kD with a predominance of low-molecular-weight proteins. The polypeptide patterns of heat-stable and thermolabile proteins differed strikingly. Heat-stable proteins accumulated in axes during the late seed maturation, comprising more than 30% of the total protein in axes of mature seeds. The polypeptide patterns of the total protein of axial organs and its particular fractions did not change in the course of seed dormancy and release. At early germination, the content of heat-stable proteins in axes decreased and their polypeptide pattern changed both in the cytosol and cell structures. We believe that at least some heat-stable proteins can function as storage proteins in the axes. Localization of storage proteins in the cells of axial organs and the role of heat-stable proteins in recalcitrant seeds are discussed.

Aesculus hyppocastanum seeds proteins dormancy germination heat-stable proteins 


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Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • N. A. Gumilevskaya
    • 1
  • M. I. Azarkovich
    • 2
  • M. E. Komarova
    • 2
  • N. V. Obroucheva
    • 2
  1. 1.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  2. 2.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

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