Russian Journal of Plant Physiology

, Volume 48, Issue 1, pp 74–83 | Cite as

Pollen Chemosensitivity to Ozone and Peroxides

  • V. V. Roshchina
  • E. V. Mel'nikova


The sensitivity of pollen from seven plant species to ozone and the stable products of ozone treatment, such as hydrogen peroxide and tert-butylhydroperoxide, was tested using pollen autofluorescence and germination as assays. Total ozone doses corresponding to 0.15–5.0 ppm (μl h) were applied. In the carotenoid-enriched pollen of Passiflora coerulea, Philadelphus grandiflorus, and Hemerocallis fulva, the treatment with low ozone doses (0.15 ppm) resulted in the disappearance of carotenoid fluorescence maxima in the region of 535–560 nm and the appearance of novel peaks, probably related to lipofuscins, in the region of 460–480 nm. Similar changes occurred one day after pollen treatment with peroxides. In the carotenoid-depleted pollen of Hippeastrum hybridum andPlantago major, the ozone treatment shifted a single peak at 480–490 nm toward the long- or short-wavelength region, depending on the ozone dose, and also changed (increased or decreased) the total fluorescence intensity. In anthocyanin-rich pollen of Papaver orientale and Petunia hybrida, neither ozone nor peroxides affected the spectrum pattern, though ozone enhanced fluorescence. Ozone and peroxides exerted opposite effects on pollen germination: ozone suppressed, whereas peroxide stimulated pollen tube growth. It is proposed that the damaging effect of ozone is not mediated by peroxide formation (which stimulates pollen germination), but rather is related to the direct oxidation of the pollen-wall components by O3 itself or by the hydroxyl radicals thereby produced.

ozone peroxides free radicals chemosensitivity 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Feder, W.A., Reduction in Tobacco Pollen Germination and Tube Elongation Induced by Low Levels of Ozone, Science, 1968, vol. 160, p. 1122.Google Scholar
  2. 2.
    Mumford, R.A., Lipke, H., Laufer, D.A., and Feder, W.A., Ozone-Induced Changes in Corn Pollen, Environ. Sci. Technol., 1972, vol. 6, pp. 427-430.Google Scholar
  3. 3.
    Nakada, M., Fukui, S., and Kanno, S., Effects of Exposure to Various Injurious Gases on Germination of Lily Pollen, Environ. Pollut., 1976, vol. 11, pp. 181-187.Google Scholar
  4. 4.
    Criege, R., Mechanism of Ozonolysis, Angew. Chem., 1975, vol. 14, pp. 745-752.Google Scholar
  5. 5.
    Hweitt, C.N., Kok, G.L., and Fall, R., Hydroperoxides in Plants Exposed to Ozone Mediate Air Pollution Damage to Alkene Emitters, Nature, 1990, vol. 344, pp. 56-58.Google Scholar
  6. 6.
    Stanley, R.G. and Linskens, H.F., Pollen. Biology, Biochemistry, Management, Berlin: Springer, 1974.Google Scholar
  7. 7.
    Roshchina, V.V., Mel'nikova, E.V., Spiridonov, N.A., and Kovaleva, L.V., Azulene as a Blue Pigment of Pollen, Dokl. Akad. Nauk, 1995, vol. 340, pp. 93-96.Google Scholar
  8. 8.
    Roshchina, V.V., Mel'nikova, E.V., and Kovaleva, L.V., Autofluorescence during Pollen–Pistil Interaction in Hippeastrum hybridum, Dokl. Akad. Nauk, 1996, vol. 349, pp. 118-120.Google Scholar
  9. 9.
    Roshchina, V.V., Mel'nikova, E.V., and Kovaleva, L.V., Changes in Fluorescece during Development of the Male Gametophyte, Fiziol. Rast. (Moscow), 1997, vol. 44, pp. 45-53 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  10. 10.
    Mel'nikova, E.V., Roshchina, V.V., and Karnaukhov, V.N., Microspectrofluorimetry of Intact Plant Pollen, Biofizika, 1997, vol. 42, pp. 226-233.Google Scholar
  11. 11.
    Roshchina, V.V., Mel'nikova, E.V., Mit'kovskaya, L.I., and Karnaukhov, V.N., Microspectrofluorimetry for the Study of Intact Plant Secretory Cells, Zh. Obshch. Biol., 1998, vol. 59, pp. 531-554.Google Scholar
  12. 12.
    Boyd, A.W., Willis, C., and Cyr, R., New Determination of Stoichiometry of the Iodometric Method for Ozone Analysis at pH 7.0, Anal. Chem., 1970, vol. 42, pp. 670-672.Google Scholar
  13. 13.
    Gutteridge, J.M., Signal Messenger and Trigger Molecules from Free Radical Reactions and Their Control by Antioxidants, Signaling Mechanisms: From Transcription Factors to Oxidative Stress, Packer, L.P. and Wirtz, K.W.A, Eds., Berlin: Springer, NATO ASI Ser., 1995, vol. H92, pp. 157-164.Google Scholar
  14. 14.
    Brooks, R.I. and Csallany, A.S., Effects of Air, Ozone, and Nitrogen Dioxide Exposure on the Oxidation of Corn and Soybean Lipids, J. Agric. Food Chem., 1978, vol. 28, pp. 1203-1209.Google Scholar
  15. 15.
    Merzlyak, M.N., Reactive Oxygen and Oxidative Processes in the Membranes of Plant Cells, Itogi Nauki Tekhn., Ser. Fiziol. Rast., 1989, vol. 6.Google Scholar
  16. 16.
    Roshchina, V.V. and Mel'nikova, E.V., The Response to the Chemosignal during Pollen–Pistil Interaction, Izv. Ross. Akad. Nauk, Ser. Biol., 1998, no. 6, pp. 678-685.Google Scholar
  17. 17.
    Hendricks, S.B. and Taylorson, R.B., Breaking of Seed Dormancy by Catalase Inhibition, Proc. Natl. Acad. Sci. USA, 1975, vol. 72, pp. 306-309.Google Scholar
  18. 18.
    Apasheva, L.M. and Komissarov, G.G., The Effect of Hydrogen Peroxide on Plant Development, Izv. Ross. Akad. Nauk, Ser. Biol., 1996, no. 5, pp. 621-623.Google Scholar
  19. 19.
    Yamasaki, H., Sakihama, Y., and Ikehara, N., Flavonoid-Peroxidase Reaction as a Detoxification Mechanism of Plant Cells against H2O2, Plant Physiol., 1997, vol. 115, pp. 1405-1412.Google Scholar
  20. 20.
    Gamalei, I.A. and Klyubin, I.V., Hydrogen Peroxide as a Signal Molecule, Tsitologiya, 1996, vol. 38, pp. 1233-1247.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • V. V. Roshchina
    • 1
  • E. V. Mel'nikova
    • 1
  1. 1.Institute of Cell BiophysicsRussian Academy of Sciences, PushchinoMoscow oblastRussia

Personalised recommendations