World Journal of Microbiology and Biotechnology

, Volume 16, Issue 7, pp 663–666

Studies on the production of extracellular protease by Alcaligenes faecalis

  • E. Berla Thangam
  • G. Suseela Rajkumar

DOI: 10.1023/A:1008989810481

Cite this article as:
Thangam, E.B. & Rajkumar, G.S. World Journal of Microbiology and Biotechnology (2000) 16: 663. doi:10.1023/A:1008989810481


Alcaligenes faecalis produced extracellular protease when incubated in media containing protein substrates. Enzyme production was found to be influenced by various culture conditions. Enzyme production was growth-associated, expressed linearity with growth and reached a maximum at the end of the growth phase. Carbohydrates and inorganic nitrogen sources could not be utilized by the bacterium for its growth, and organic nitrogen appeared to be a primary determinant in protease production. Enzyme production reached its maximum level of 171.2 U/ml when the culture was incubated at 30 °C at pH 8.0. Ca2+ and Mg2+ enhanced the enzyme production. The crude enzyme powder was stable at high alkaline pH and stable upto 6 months at the storage temperature of 0–4 °C.

Alcaligenes faecalis batch culture extracellular protease 

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • E. Berla Thangam
    • 1
  • G. Suseela Rajkumar
    • 1
  1. 1.Microbiology DivisionCentral Leather Research InstituteChennaiIndia

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