Biotechnology Techniques

, Volume 13, Issue 12, pp 909–913 | Cite as

Continuous high-pressure disruption of marine diatom Haslea ostrearia. Assessment by laser diffraction particle sizer

  • Nathalie Rossignol
  • Richard Moan
  • Pascal Jaouen
  • Jean-Michel Robert
  • Francis Quéméneur

Abstract

In order to recover the intracellular blue-green pigment, marennine, synthesized by the microalga Haslea ostrearia, a continuous flow high-pressure disrupter was evaluated in the range: 30–270 MPa. Cells were partly broken from 30 MPa, but a pressure of 100 MPa (1 cycle) was required to obtain optimal pigment release. The latter was directly linked to the physical cell breakage dependent upon the applied pressure and the number of disintegration cycles. Granulometric analysis by laser diffraction technology (0.04–2000 μm) revealed a size reduction of cell fragments when increasing these two operating parameters.

Haslea ostrearia high-pressure disrupter laser diffraction pigment release 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Nathalie Rossignol
    • 1
  • Richard Moan
    • 2
  • Pascal Jaouen
    • 1
  • Jean-Michel Robert
    • 2
  • Francis Quéméneur
    • 1
  1. 1.Laboratoire de Génie des Procédés – CRTTInstitut des Substances et Organismes de la Mer – ISOMerSaint-Nazaire CedexFrance
  2. 2.Laboratoire de Biologie MarineInstitut des Substances et Organismes de la Mer – ISOMer, Faculté des Sciences et des TechniquesNantes Cedex 3France

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