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Biotechnology Techniques

, Volume 13, Issue 4, pp 277–278 | Cite as

A modified AFLP with fluorescence-labelled primers and automated DNA sequencer detection for efficient fingerprinting analysis in plants

  • Junchao Huang
  • Mei Sun
Article

Abstract

A modified AFLP (amplified fragment length polymorphism) technique is described. Fluorescence-labelled primers were used in the selective amplifications. The amplified fragments were detected on denaturing polyacrylamide gels using an automated ALF DNA sequencer with the fragment option. The modified AFLP technique avoids the use of isotopes or silver staining, but gives a much higher resolution than other AFLP detection systems.

AFLP amplified fragment length polymorphism DNA fingerprinting 

References

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Junchao Huang
    • 1
  • Mei Sun
    • 1
  1. 1.Department of ZoologyThe University of Hong KongHong Kong

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