Transformation of Penicillium expansum with a heterologous gene which confers resistance to benomyl

  • Eustáquio Souza Dias
  • Marisa Vieira de Queiroz
  • Patrícia Gomes Cardoso
  • Everaldo Gonçalves de Barros
  • Elza Fernandes Araújo
Article

Abstract

Transformation of Penicillium expansum with the pBT6 vector yielded 8 to 34 transformants per μg of DNA. Hybridization analyses revealed that homologous recombination occurred in most of the transformants. Twenty-one out of 25 transformants analysed showed hybridization patterns which were indistinguishable from that of the wild type.

Benomyl heterologous transformation Penicillium β-tubulin 

References

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Eustáquio Souza Dias
    • 1
  • Marisa Vieira de Queiroz
    • 2
  • Patrícia Gomes Cardoso
    • 2
  • Everaldo Gonçalves de Barros
    • 3
  • Elza Fernandes Araújo
    • 2
  1. 1.Departamento de BiologiaUniversidade Federal de LavrasLavras, MGBrasil
  2. 2.Departamento de MicrobiologiaUniversidade Federal de ViçosaViçosa, MGBrasil
  3. 3.Departamento de Biologia GeralUniversidade Federal de ViçosaViçosa, MGBrasil

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