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Effect of various carbon and nitrogen sources on cellulose synthesis by Acetobacter xylinum

  • K.V. Ramana
  • A. Tomar
  • Lokendra Singh
Article

Abstract

The effect of various carbon and nitrogen sources on cellulose membrane production by Acetobacter xylinum was evaluated. Among the carbon sources, sucrose, glucose and mannitol were found to be suitable for optimum levels of cellulose production. The strain was able to utilize a wide range of protein and nitrogen sources such as peptone, soybean meal, glycine, casein hydrolysate, and glutamic acid for cellulose synthesis. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of pellicle proteins (PP) revealed electrophoretic bands of molecular masses in the range of 116–20 kDa. Furthermore, the strain can be useful for the removal of various nitrogenous and carbon substrates present in waste waters.

Acetobacter xylinum biotechnology carbon source cellulose membrane immobilization 

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References

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Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • K.V. Ramana
    • 1
  • A. Tomar
    • 1
  • Lokendra Singh
    • 1
  1. 1.Biotechnology DivisionDefence R & D EstablishmentGwaliorIndia

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