Biotechnology Techniques

, Volume 13, Issue 11, pp 771–779

Comparison of DNA extraction methods for PCR amplification of mitochondrial cytochrome c oxidase subunit II (COII) DNA from primate fecal samples

  • Christopher A. Whittier
  • Arun K. Dhar
  • Chip Stem
  • Jane Goodall
  • Acacia Alcivar-Warren
Article

Abstract

Mitochondrial COII DNA was amplified by PCR from total DNA extracted from field collected primate fecal samples (n=24) which had been stored without refrigeration for over 30 days. High molecular weight DNA total DNA was obtained from samples stored in 70% (v/v) ethanol, SDS lysis buffer (LB) and guanidine isothiocyanate buffer (GTB) than from samples stored in 10% formalin. Fecal DNA quality and COII amplification varied according to storage solution (formalin, ethanol, LB and GTB), extraction method (LB-based and GTB-based) and primate species (chimpanzee, baboon, human). It is recommended that fecal samples be collected in LB for DNA analysis. However, GTB-based protocols are suitable when total RNA is needed for epidemiological studies of viral diseases or gene expression analysis.

baboon chimpanzee COII DNA fecal human 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Christopher A. Whittier
    • 1
  • Arun K. Dhar
    • 1
  • Chip Stem
    • 2
  • Jane Goodall
    • 3
  • Acacia Alcivar-Warren
    • 1
  1. 1.Department of Environmental and Population Health andColumbia
  2. 2.Section of International MedicineTufts University School of Veterinary MedicineNorth GraftonUSA
  3. 3.Jane Goodall Institute-USARidgfieldUSA

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