A probing feeding strategy for Escherichia coli cultures
A strain-independent feeding strategy for fed-batch cultures of Escherichia coli is presented. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.
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- Åkesson M, Nordberg Karlsson E, Hagander P, Axelsson JP, Tocaj A (1999) On-line detection of acetate formation in Escherichia coli cultures using dissolved oxygen responses to feed transients. Biotechnol. Bioeng., in press.Google Scholar
- Andersen K, von Meyenburg K (1980) Are growth rates of Escherichia coli in batch cultures limited by respiration? J. Bacteriol 144: 114-123.Google Scholar
- Bauer K, Ben-Bassat A, Dawson M, de la Puente V, Neway J (1990) Improved expression of human interleukin-2 in high-cell-density fermentor cultures of Escherichia coli K-12 by a phosphotransacetylase mutant. Appl. Environ. Microbiol. 56: 1296-1302.Google Scholar
- Bech Jensen E, Carlsen S (1990) Production of recombinant human growth hormone in Escherichia coli: expression of different precursors and physiological effects of glucose, acetate and salts. Biotechnol. Bioeng. 36: 1-11.Google Scholar
- Lee SY (1996) High cell-density culture of Escherichia coli. TIBTECH 14: 98-105.Google Scholar
- Luli GW, Strohl WR (1990) Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl. Environ. Microbiol. 56: 1004-1011.Google Scholar
- Qiu J, Swartz JR, Georgiou G (1998) Expression of active human tissue-type plasminogen activator in Escherichia coli. Appl. Environ. Microbiol. 64: 4891-4896.Google Scholar
- Reiling HE, Laurila H, Fiechter A (1985) Mass culture of Escherichia coli: medium development for low and high density cultivation of Escherichia coli B/r in minimal and complex media. J. Biotechnol. 2: 191-206.Google Scholar
- Xu B, Jahic M, Enfors S-O (1999) Modeling of overflow metabolism in batch and fed-batch cultures of Escherichia coli. Biotechnol. Prog. 15: 81-90.Google Scholar